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  • 研究・開発
  • 細胞分析
  • Cellular Imaging & Analysis
  • Cellular Imaging Reagents
  • PhenoVue Fluorescent Secondary Antibodies
  • PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed

PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed

PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed View All
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed

Highly cross-adsorbed donkey anti-mouse IgG (H+L) antibody conjugated with PhenoVue™ Fluor 488.

PhenoVue Fluor 488 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in imaging microscopy and high-content screening applications.

Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 488 donkey anti-mouse antibody, highly cross-adsorbed, has a maximum excitation wavelength of 495nm and a maximum emission wavelength of 520nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Feature Specification
Color Green
Filter FITC
Fluorophore PhenoVue™ Fluor 488
Host Species Donkey
Target Species Mouse

Highly cross-adsorbed donkey anti-mouse IgG (H+L) antibody conjugated with PhenoVue™ Fluor 488.

PhenoVue Fluor 488 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in imaging microscopy and high-content screening applications.

Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 488 donkey anti-mouse antibody, highly cross-adsorbed, has a maximum excitation wavelength of 495nm and a maximum emission wavelength of 520nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information
Product variant
Quantity: 1 x 1 mg
Part #:
2DXM488H1
For research use only. Not for use in diagnostic procedures.
Request more information
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
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Product information

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  • Additional product information
  • Specifications
  • Image gallery
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Overview

PhenoVue Fluor 488 - donkey anti-mouse, IgG (H+L) antibody, highly cross-adsorbed, has been cross-adsorbed against various IgG species such as rat, mouse, hamster, human, bovine, horse, or chicken serum to minimize cross-reactivity with other species. Specificity and sensitivity are improved and background reduced with PhenoVue Fluor 488 - donkey anti-mouse IgG (H+L) antibody, highly cross-adsorbed.

It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Additional product information

Features
Numbers of vials per unit 1
Quantity or volume per unit 1 mg
Form Lyophilized
Storage 2-8°C
Recommended working concentration 1.5 µg/mL (10nM)
Maximum excitation wavelength 495 nm
Maximum emission wavelength 520 nm
Common filter set FITC
Live cell staining No
Fixed cell staining Yes
Equivalent number of microplates 25-70 x 96-well microplates
20-70 x 384-well microplates
35-90 x 1536-well microplates

 

Specifications

Color
Green
Form
Lyophilized
Maximum Emission Wavelength (Emmax)
520 nm
Maximum Excitation Wavelength (Exmax)
495 nm
Application
High Content Imaging
Microscopy
Brand
PhenoVue™
Cross-Adsorption
Highly cross-adsorbed
Detection Modality
Fluorescence
Filter
FITC
Fluorophore
PhenoVue™ Fluor 488
Host Species
Donkey
Quantity
1 x 1 mg
Sample Type
Fixed samples only
Shipping Conditions
Shipped Ambient
Storage Conditions
2-8 °C, protected from light
Target Species
Mouse
Type
Individual reagent

Image gallery

PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Mouse Antibody Highly Cross-Adsorbed

Spectra viewer

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PhenoVue Cellular Imaging Reagents Flyer

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PhenoVue reagents & imaging microplates product list

PhenoVue reagents & imaging microplates product list

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