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PhenoVue Autophagy Staining Kit (LC3, p62/SQSTM1, LAMP1) for 2 x 96-well plates
PhenoVue Autophagy Staining Kit (LC3, p62/SQSTM1, LAMP1) for 2 x 96-well plates
Hydroxychloroquine-treated HeLa cells stained with the PhenoVue autophagy staining kit.
Product variant
Quantity: 1 kit
Part #:
PAUT14
For research use only. Not for use in diagnostic procedures.
PhenoVue Autophagy Staining Kit (LC3, p62/SQSTM1, LAMP1) for 2 x 96-well plates
Hydroxychloroquine-treated HeLa cells stained with the PhenoVue autophagy staining kit.
PhenoVue Autophagy Staining Kit (LC3, p62/SQSTM1, LAMP1) for 2 x 96-well plates
Product information
Overview
The PhenoVue autophagy staining kit provides a powerful and user-friendly solution for high-content imaging of key autophagy markers in fixed cells. It includes characterized, specific antibodies against:
- LC3 (microtubule-associated proteins 1A/1B light chain 3B) which is involved in autophagosome membrane formation and elongation.
- p62/SQSTM1, a selective cargo adaptor that accumulates when autophagy is impaired, serving as a readout for autophagic degradation.
- LAMP1 (lysosome-associated membrane protein 1), a marker of lysosomes and autolysosomes involved in final cargo degradation.
The kit also includes PhenoVue Fluor secondary antibody conjugates, fixation/permeabilization buffers and a streamlined protocol designed for easy integration into high-content imaging workflows.
The kit is designed for optimal performance with Revvity’s PhenoPlate™ imaging microplates and has been verified on high-content screening systems like the Opera Phenix™ Plus, providing consistent results and experimental flexibility for advanced autophagy research.
Kit components /quantity
- PhenoVue Hoechst 33342 Nuclear stain / 1 vial of 70 μL (500x)
- PhenoVue anti-p62/SQSTM1 mouse IgG1 antibody Human only / 1 vial of 100 μL (100x)
- PhenoVue anti-LAMP1 rat IgG2b antibody Human only / 1 vial of 100 μL (100x)
- PhenoVue anti-LC3 rabbit IgG antibody Human, mouse and rat / 1 vial of 100 μL (100x)
- PhenoVue Fluor 488 - Goat anti-mouse antibody, highly cross adsorbed / 1 vial of 100 μL (100x)
- PhenoVue Fluor 555 - Goat anti-rat antibody, highly cross adsorbed / 1 vial of 100 μL (100x)
- PhenoVue Fluor 647 - Goat anti-rabbit antibody, highly cross adsorbed / 1 vial of 100 μL (100x)
- PhenoVue Paraformaldehyde 4% solution / 1 vial of 25 mL (1x)
- PhenoVue Permeabilization 0.5% Triton X-100 solution / 1 vial of 25 mL (5x)
- PhenoVue Dye diluent A / 1 vial of 8 mL (5x)
Specifications
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Assay Points |
2 x 96-well microplates
|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Host Species |
Mouse
|
| Product Group |
Kit
|
| Quantity |
1 kit
|
| Sample Type |
Fixed cells only
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Type |
Kit
|
Resources
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Product Note
PhenoVue anti-p62/SQSTM1 mouse IgG1 antibody product information sheet
Instructions for use and verification data for PhenoVue anti-p62/SQSTM1 antibody
Product Note
PhenoVue autophagy staining kit product information sheet
Instructions for use and verification data for PhenoVue autophagy staining kit
Flyer
PhenoVue reagents & imaging microplates product list
PhenoVue reagents & imaging microplates product list
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