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PhenoVue Autophagy Staining Kit (LC3, p62/SQSTM1, LAMP1) for 2 x 96-well plates
PhenoVue Autophagy Staining Kit (LC3, p62/SQSTM1, LAMP1) for 2 x 96-well plates
Hydroxychloroquine-treated HeLa cells stained with the PhenoVue autophagy staining kit.
The PhenoVue autophagy staining kit comprises three highly characterized antibodies against LC3, p62/SQSTM1, and LAMP1 plus Hoechst for nuclei staining.
It also contains PhenoVue Fluor secondary antibody conjugates and fixation/permeabilization buffers. With a simplified protocol, the kit enables specific multiplexed detection of autophagy-related structures. The kit's performance has been verified on high-content screening systems such as the Opera Phenix Plus.
View our extensive performance and verification data in the Product Information Sheet via the Resources tab below.
The PhenoVue autophagy staining kit comprises three highly characterized antibodies against LC3, p62/SQSTM1, and LAMP1 plus Hoechst for nuclei staining.
It also contains PhenoVue Fluor secondary antibody conjugates and fixation/permeabilization buffers. With a simplified protocol, the kit enables specific multiplexed detection of autophagy-related structures. The kit's performance has been verified on high-content screening systems such as the Opera Phenix Plus.
View our extensive performance and verification data in the Product Information Sheet via the Resources tab below.
Product variant
Quantity: 1 kit
Part #:
PAUT14
For research use only. Not for use in diagnostic procedures.
PhenoVue Autophagy Staining Kit (LC3, p62/SQSTM1, LAMP1) for 2 x 96-well plates
Hydroxychloroquine-treated HeLa cells stained with the PhenoVue autophagy staining kit.
PhenoVue Autophagy Staining Kit (LC3, p62/SQSTM1, LAMP1) for 2 x 96-well plates
Product information
Overview
The PhenoVue autophagy staining kit provides a powerful and user-friendly solution for high-content imaging of key autophagy markers in fixed cells. It includes characterized, specific antibodies against:
- LC3 (microtubule-associated proteins 1A/1B light chain 3B) which is involved in autophagosome membrane formation and elongation.
- p62/SQSTM1, a selective cargo adaptor that accumulates when autophagy is impaired, serving as a readout for autophagic degradation.
- LAMP1 (lysosome-associated membrane protein 1), a marker of lysosomes and autolysosomes involved in final cargo degradation.
The kit also includes PhenoVue Fluor secondary antibody conjugates, fixation/permeabilization buffers and a streamlined protocol designed for easy integration into high-content imaging workflows.
The kit is designed for optimal performance with Revvity’s PhenoPlate™ imaging microplates and has been verified on high-content screening systems like the Opera Phenix™ Plus, providing consistent results and experimental flexibility for advanced autophagy research.
Kit components /quantity
- PhenoVue Hoechst 33342 Nuclear stain / 1 vial of 70 μL (500x)
- PhenoVue anti-p62/SQSTM1 mouse IgG1 antibody Human only / 1 vial of 100 μL (100x)
- PhenoVue anti-LAMP1 rat IgG2b antibody Human only / 1 vial of 100 μL (100x)
- PhenoVue anti-LC3 rabbit IgG antibody Human, mouse and rat / 1 vial of 100 μL (100x)
- PhenoVue Fluor 488 - Goat anti-mouse antibody, highly cross adsorbed / 1 vial of 100 μL (100x)
- PhenoVue Fluor 555 - Goat anti-rat antibody, highly cross adsorbed / 1 vial of 100 μL (100x)
- PhenoVue Fluor 647 - Goat anti-rabbit antibody, highly cross adsorbed / 1 vial of 100 μL (100x)
- PhenoVue Paraformaldehyde 4% solution / 1 vial of 25 mL (1x)
- PhenoVue Permeabilization 0.5% Triton X-100 solution / 1 vial of 25 mL (5x)
- PhenoVue Dye diluent A / 1 vial of 8 mL (5x)
Specifications
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Assay Points |
2 x 96-well microplates
|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Host Species |
Mouse
|
| Product Group |
Kit
|
| Quantity |
1 kit
|
| Sample Type |
Fixed cells only
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Type |
Kit
|
Resources
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Product Note
PhenoVue anti-p62/SQSTM1 mouse IgG1 antibody product information sheet
Instructions for use and verification data for PhenoVue anti-p62/SQSTM1 antibody
Product Note
PhenoVue autophagy staining kit product information sheet
Instructions for use and verification data for PhenoVue autophagy staining kit
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PhenoVue reagents & imaging microplates product list
PhenoVue reagents & imaging microplates product list
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