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PhenoVue 505 Live Cell Caspase-3/7 Activity Stain

PhenoVue™ 505 live cell caspase-3/7 activity stain is a no wash, cell permeable fluorogenic dye which is specifically cleaved by activated pro apoptotic caspases 3 and 7 in living cells, leading to fluorescence increase.

Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue 505 live cell caspase-3/7 activity stain, has a maximum excitation wavelength of 505 nm and a maximum emission wavelength of 530 nm.

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information

Feature	Specification
Cell Function Indicator	Apoptosis indicator
Color	Green
Filter	FITC

View our extensive validation data in the Product Information Sheet within the Resources tab below.

View product information

Product variant

Quantity: 1 x 100 µL

Part #:

CP22G1

For research use only. Not for use in diagnostic procedures.

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Product information

Overview

PhenoVue 505 live cell caspase-3/7 activity stain is a no wash, cell permeable fluorogenic dye which is specifically cleaved by activated pro apoptotic caspases 3 and 7 in living cells, leading to fluorescence increase.

Sensitive, rapid and photostable, PhenoVue 505 live cell caspase-3/7 activity stain exhibits green emission and can be multiplexed with blue, orange and red colors.

PhenoVue 505 live cell caspase-3/7 activity stain uses a DEVD substrate conjugated with a non-fluorescent cell permeable DNA binding dye which becomes fluorescent when cells undergo apoptosis.

Typical working concentration: 5 µM

Equivalent number of microplates:

0.5 - 2 x 96-well microplates
0.5 - 2 x 384-well microplates
1 - 3 x 1536-well microplates

Specifications

Physical specifications

Color	Green
Form	1 mM solution in DMSO
Maximum Emission Wavelength (Em_max)	530 nm
Maximum Excitation Wavelength (Ex_max)	505 nm

Other specifications

Application	High Content Imaging Microscopy
Brand	PhenoVue™
Cell Function Indicator	Apoptosis indicator
Detection Modality	Fluorescence
Filter	FITC
Quantity	1 x 100 µL
Sample Type	Live samples only
Shipping Conditions	Shipped in Dry Ice
Storage Conditions	-16 °C or below, protected from light
Type	Individual reagent