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PhenoVue 492 Live Cell Tracer Stain
PhenoVue 492 Live Cell Tracer Stain
Representative images of HaCaT cells stained with PhenoVue 492 live cell tracer (5 µM, 45 min) to monitor wound healing. Time-lapse acquisition on the Operetta CLS™ high-content screening system.
PhenoVue™ 492 live cell tracer stain is a cell-permeable, non-fluorescent probe for long-term live-cell labeling. After passive entry into cells, the dye is activated by intracellular esterases, yielding a brightly fluorescent and membrane-impermeant form. Once converted, it reacts with intracellular thiol groups on proteins, generating stable covalent labeling that enables durable signal retention throughout extended live-cell experiments.
| Feature | Specification |
|---|---|
| Color | Green |
| Filter | FITC |
| Quantity | 1 |
PhenoVue™ 492 live cell tracer stain is a cell-permeable, non-fluorescent probe for long-term live-cell labeling. After passive entry into cells, the dye is activated by intracellular esterases, yielding a brightly fluorescent and membrane-impermeant form. Once converted, it reacts with intracellular thiol groups on proteins, generating stable covalent labeling that enables durable signal retention throughout extended live-cell experiments.
Product variant
Unit Size: 0.5 mg
Part #:
CP23G1
For research use only. Not for use in diagnostic procedures.
PhenoVue 492 Live Cell Tracer Stain
Representative images of HaCaT cells stained with PhenoVue 492 live cell tracer (5 µM, 45 min) to monitor wound healing. Time-lapse acquisition on the Operetta CLS™ high-content screening system.
PhenoVue 492 Live Cell Tracer Stain
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Product information
Overview
PhenoVue Fluor 492 Live cell tracer stain allows tracking of live cells for extended periods (up to 50-72 hours), after a short loading period of 15-45 minutes, without detectable cytotoxicity. The dye is highly photostable, is inherited by daughter cells during division, and provides bright green fluorescence. Its spectral properties make it compatible for multiplexing with blue, orange, and red fluorophores, offering flexibility for multicolor imaging applications.
View our validation data in the Product Information Sheet within the Resources tab below.
Specifications
| Color |
Green
|
|---|---|
| Form |
Dried
|
| Maximum Emission Wavelength (Emmax) |
519 nm
|
| Maximum Excitation Wavelength (Exmax) |
489/492 nm
|
| Application |
High Content Imaging
Microscopy
|
|---|---|
| Assay Points |
20 x 96-well microplates
|
| Brand |
PhenoVue™
|
| Detection Modality |
Fluorescence
|
| Filter |
FITC
|
| Product Group |
Organelle and cell compartment
|
| Quantity |
1
|
| Sample Type |
Live cells only
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Storage Conditions |
-16° C or below, protected from light
|
| Type |
Individual reagent
|
| Unit Size |
0.5 mg
|
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Resources
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