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  • Opera Phenix Plus ハイコンテントスクリーニングシステム - シングルカメラ

Opera Phenix Plus ハイコンテントスクリーニングシステム - シングルカメラ

Opera Phenix Plus シングルカメラシステムは、バイオ医薬品スタートアップ企業やアカデミア研究室の研究環境や予算に配慮して構成されたハイコンテントアナリシスシステムです。

革新性とコストバランスを両立したハイコンテントシステムとして、研究および創薬研究を支援します。

マルチカメラ構成の詳細については、Opera Phenix Plus システムのページをご覧ください。

本製品は研究用です。診断用にはご使用いただけません。
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revvity opera phenix plus image 700x700
"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.
revvity opera phenix plus image 700x700
"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.
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Opera Phenix Plus ハイコンテントスクリーニングシステム - シングルカメラ
revvity opera phenix plus image 700x700
"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.
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revvity opera phenix plus image 700x700
"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.
revvity opera phenix plus image 700x700
Opera Phenix Plus ハイコンテントスクリーニングシステム - シングルカメラ
revvity opera phenix plus image 700x700
"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.
revvity opera phenix plus image 700x700
"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.
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Part #: HH14002001
Imaging Modality:
明視野, 共焦点, デジタル位相差, 蛍光
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Overview

Opera Phenix Plusでさらに深まる細胞への理解

より多くの知見: ハハイコンテントアナリシスは、これまでの細胞学的手法より、より深く広範な情報を得られるため、細胞応答を詳細に記述することを可能にします。

細部まで定量化: 単一細胞から細胞集団全体まで、表現型の変化を様々なスケールで解析します。ハイコンテントアナリシスが細部までの定量を可能にします。

3Dイメージングの強化: 水浸対物レンズにより3D画質が向上し、複雑な生物学的構造内の微細な変化を明らかにします。

スループットの向上: 複数台のカメラで撮像速度と作業効率が向上します。ハイコンテントアナリシスは研究の速度を加速します。

Revvityの先進的なハイコンテントアナリシスソリューションで、価値ある成果をより早く得られます。

Additional product information

Applications
Micro Physiological Systems (MPS)

Micro Physiological Systems (MPS)

より鮮明な画像から重要な特徴やプロセスが観察・定量できるため、3D細胞モデルの理解がより多面的に深まります。

Functional genomic screening

Functional genomic screening

一回の実験で数千の遺伝子操作に対する細胞応答を表現型解析できます。形態変化と遺伝的エビデンスの結びつけが容易に実現します。

Cell painting

Cell painting

キット化された試薬と解析メソッドにより、マルチパラメトリックスクリーニングを簡素化し、複雑さの低減と研究に集中するための時間を確保できます。

Drug sensitivity testing

Drug sensitivity testing

薬剤処理に対するex vivoでの細胞機能を迅速に特性評価することで、合理的な治療選択レジメンを決定できます。

Features at a glance
 

microlens pinhole disk

マイクロレンズ強化ピンホールディスク

マイクロレンズにより励起効率が向上し、広いピンホール間隔は、共焦点イメージングにおけるアウトフォーカス光を低減します。

automated water immersion objectives

自動水浸対物レンズ

シグナルを強化し、より多くの光を取り込みながらZ軸分解能を向上させることで、画像とデータの品質を改善します。

machine learning

マシンラーニング

Revvity独自のPhenoLOGICマシンラーニング技術を使用すると、画像解析の専門知識がなくても容易に解析アルゴリズムを作成できます。

 

intelligent image acquisition

インテリジェント画像取得

関心のある対象のみを高倍率で撮像できます。撮像時間とデータサイズを大幅に削減できます。

powerfully simple analysis

高機能かつシンプルな解析

Harmony™ハイコンテントイメージングおよび解析ソフトウェアは、すぐに使用できるテンプレートとシンプルな手順を備えます。解析のカスタマイズも簡単に実施できます。

3d analysis

3D解析

3DビューおよびXYZビューアで細胞モデルを可視化し、体積などたくさんの3D関連表現型を定量化できます。

 

multiple cameras

アップグレード対応

同時撮像を可能にするため、最大3台のカメラを追加できます。

 

Opera Phenix Plusファミリー：代表的な構成 

Opera Phenix Plus Single
 

Phenixファミリーの他の構成と同等の感度と分解能を備えています。後からカメラを追加しアップグレードできます。

Opera Phenix Plus Simultaneous

マルチカラーの同時共焦点画像取得と高速なマルチプレックス解析に最適な、高速デュアルカメラシステムです。

Opera Phenix Plus FRET
 

5本のレーザーと4台のカメラ構成により、CFP/YFP FRETアプリケーションに対応し、タンパク質間相互作用の大規模なマッピングを可能にします。

Opera Phenix Plus Screener

最大限のスループットと性能を実現する構成です。4台のカメラと高出力レーザーを搭載し、大規模ライブラリーのスクリーニングを実現します。

 

    Single
System Options カメラの台数 1
カメラのアップグレードパス 2台または4台
自動水浸対物レンズ ✔
透過光 ✔
環境制御 ✔
オンボードリキッドハンドリング optional
ロボティクス／オートメーション対応 ✔
エミッションフィルター 8
Acquisition speed 2D/3D +/+
Lasers 375/425 nm -
405 nm ✔
488 nm ✔
561 nm ✔
640 nm ✔
Imaging modes 同時撮像時のクロストークを最小化するSynchrony光学系 -
マルチカラー蛍光イメージング ✔
マルチカラー同時共焦点イメージング -
3Dイメージング ✔
明視野およびデジタル位相差 ✔
蛍光広視野イメージング ✔
レシオメトリックFRET* +
高速フレームレートイメージング ✔

*CFP/YFP or spectrally similar pairs

 

画像取得および解析の簡素化
  • 直感的なワークフロー：Harmony™ソフトウェアは、画像取得から解析、評価までをガイドする直感的なユーザーインターフェースを提供します。
  • セットアップ用テンプレート：テンプレートを使用することで、取得チャネルや各種パラメーターを効率的に設定できます。
  • Ready-made solutions: 一般的な画像解析タスク向けに事前構築された解析シークエンスを適用し、ワークフローを簡素化できます。
  • カスタマイズ可能なビルディングブロック：画像解析用ビルディングブロックを用いて、個々のアプリケーションに合わせた解析シークエンスを作成、設定、カスタマイズできます。
  • 高度な解析機能：Harmony™には、テクスチャ解析やSTAR形態解析などの高度な解析機能が含まれており、細胞形態の詳細な記述や表現型の高い識別性を実現します。
  • データ管理：ソフトウェアは、解析結果およびメタデータ（アッセイレイアウト、装置設定、ユーザー定義のキーワードや注釈を含む）を自動的に保存します。
検証済みソリューションによる効率的な研究推進
  • ハイコンテントイメージング用マイクロプレート：
    • ハイコンテントイメージング用途で最適な性能を発揮するよう設計されたPhenoPlate™384ウェルマイクロプレート
    • 3DモデルのイメージングにはCellCarrier Spheroid ULAプレート
  • 自動化およびワークステーション:
    • Opera Phenix Plusシステムを自動化することで、スループットと生産性を向上させ、ばらつきおよび試薬コストを低減
    • セルペインティング、3D細胞培養、表現型スクリーニング向けに、explorer™G3ワークステーションを用いた自動化ワークフローを活用
  • 効率的なデータ管理:
    • 高性能コンピューティングと業界標準のオブジェクトストレージを使用したImage Artist™プラットフォームへ、解析結果を自動的にエクスポートし、スケーラブルなマルチユーザー対応の画像解析および管理を実現
    • Opera Phenix Plusおよびその他のハイコンテントスクリーニング(HCS)システムで取得したすべての細胞画像データにアクセスし、再解析、保存、共有が可能
  • Signals Oneによる高度な解析:
    • スクリーニングデータの解析およびバリデーションを実施
    • QC解析、信頼性の高い正規化、マルチバリアントヒット層別化、用量反応曲線および薬剤応答プロファイリングを実施

Specifications

Dimensions 134.0 cm (W) x 65.0 cm (D) x 47.0 cm (H)
Automation Compatible
Yes
Brand
Opera Phenix Plus
Imaging Modality
明視野
共焦点
デジタル位相差
蛍光
Unit Size
1 unit

Image gallery

"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.
"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.
Opera Phenix Plus ハイコンテントスクリーニングシステム - シングルカメラ
"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.
"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.
Opera Phenix Plus ハイコンテントスクリーニングシステム - シングルカメラ
"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.
"Organoids from pediatric brain tumor imaged on the Opera Phenix Plus high-content screening system. "
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
Arabidopsis cells imaged on the Opera Phenix high-content screening system.
HepG2 spheroids imaged on the Opera Phenix Plus high-content screening system.
Human iPSC-derived neurons imaged on the Opera Phenix Plus high-content screening system.
Neuroblastoma SHSY5Y cells imaged on the Opera Phenix Plus high-content screening system.
HAP1 cell pool imaged on the Opera Phenix Plus high-content screening system.
HepG2 cells imaged on the Opera Phenix high-content screening system.
HeLa cells imaged on the Opera Phenix Plus high-content imaging system.
Induced pluripotent stem cells imaged on the Opera Phenix high-content screening system.
Immortalized human podocytes imaged on the Opera Phenix Plus high-content screening system.
5-plex phenotypic assay imaged on the Opera Phenix Plus high-content screening system.
A549 cells on a Semarion SemaCyte microcarrier imaged on the Opera Phenix Plus high-content screening system.
Neurons imaged on the Operetta CLS high-content analysis system.

Video gallery

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Opera Phenix Plus ハイコンテントスクリーニングシステム - シングルカメラ
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Opera Phenix Plus ハイコンテントスクリーニングシステム - シングルカメラ
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