NEXTFLEX NGS Cleanup Beads

NEXTFLEX NGS Cleanup Beads are SPRI-based magnetic beads used to purify DNA fragments during NGS library prep or PCR clean-up. They bind DNA (selectively by size) and remove primers, nucleotides, salts, and adapters. Use them in protocols requiring DNA cleanup or size selection.

NEXTFLEX beads are designed as a drop-in replacement for Beckman Coulter’s AMPure XP. In head-to-head tests, they deliver comparable DNA recovery and size-selection performance at a much lower price. Users can follow the same protocols (including bead-to-sample ratios) for PCR products or NGS library cleanup.

Yes. By adjusting the bead-to-sample ratio, NEXTFLEX beads can perform DNA size selection (e.g. enriching or depleting fragments above or below certain sizes). Revvity’s data shows predictable, consistent size cuts similar to AMPure XP or SPRIselect.

SPRI is a DNA purification method using paramagnetic beads coated with a reversible DNA-binding chemistry. When mixed with DNA and a binding buffer, the beads capture nucleic acids. A magnet pulls the beads (with DNA) aside for washing, then DNA is eluted. SPRI beads enable high recovery and scalable cleanup without centrifugation.

These beads efficiently recover double-stranded DNA fragments >100 bp, with typical recoveries of 70–90% for fragments above 100 bp. By adjusting the reagent ratio, you can target different size ranges (small fragments or large fragment selection) for sequencing libraries.

Yes. The beads are fully compatible with standard liquid-handling platforms (e.g. robotic workstations). They work both manually and on automation, offering the same SPRI cleanup protocol.