JP
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
NEXTFLEX Rapid XP v3 DNA-Seq Kit for Metagenomics
NEXTFLEX Rapid XP v3 DNA-Seq Kit for Metagenomics
Robust library prep kit that generates high-complexity libraries for NGS on Illumina and Element platforms.
The NEXTFLEX™ Rapid XP v3 DNA-Seq Kit for Metagenomics provides fast, enzymatic fragmentation-based library preparation for shotgun sequencing of microbial communities on Illumina® and Element Biosciences® platforms. The workflow supports DNA inputs from <1 ng to 500 ng, with a PCR-free option at ≥75 ng. Each purchase includes access to Cosmos-Hub®, providing taxonomic and functional analysis pipelines for shotgun metagenomics.
| Feature | Specification |
|---|---|
| Automation Compatible | Yes |
The NEXTFLEX™ Rapid XP v3 DNA-Seq Kit for Metagenomics provides fast, enzymatic fragmentation-based library preparation for shotgun sequencing of microbial communities on Illumina® and Element Biosciences® platforms. The workflow supports DNA inputs from <1 ng to 500 ng, with a PCR-free option at ≥75 ng. Each purchase includes access to Cosmos-Hub®, providing taxonomic and functional analysis pipelines for shotgun metagenomics.
Product variants
Unit Size: 8 rxns
Part #:
NOVA-5249-21S
Unit Size: 48 rxns
Part #:
NOVA-5249-22S
Unit Size: 96 rxns
Part #:
NOVA-5249-23S
For research use only. Not for use in diagnostic procedures.
NEXTFLEX Rapid XP v3 DNA-Seq Kit for Metagenomics
Robust library prep kit that generates high-complexity libraries for NGS on Illumina and Element platforms.
Loading...
Product information
Overview
The NEXTFLEX Rapid XP v3 DNA-Seq Kit for Metagenomics combines fast, automation-ready library preparation with integrated downstream analysis. Designed for shotgun metagenomic sequencing of microbial communities, each purchase includes access to Cosmos-Hub® for streamlined analysis and interpretation.
- Combined enzymatic fragmentation, end repair, and A-tailing; sequencing-ready libraries in as little as 2.5 hours
- DNA input range from <1 ng to 500 ng; PCR-free option at ≥75 ng
- Included Cosmos-Hub access for taxonomic and functional shotgun metagenomics analysis
- CHAMP and KEPLER profilers for human-associated and host-agnostic microbiome analysis
- Up to 1,536 UDIs available for high-throughput multiplexing
- Automation-ready for manual and automated workflows
- Compatible with Illumina and Element Biosciences sequencing platforms
Additional product information
Analysis Powered by Cosmos-Hub®
The purchase of the NEXTFLEX Rapid XP v3 DNA-Seq Kit for Metagenomics includes access to Cosmos-Hub®, a secure, cloud-based analysis platform for WGS metagenomics. Cosmos-Hub® enables taxonomic and functional profiling, antimicrobial resistance gene detection, and microbiome diversity analysis through version-controlled pipelines.
Key features:
- Taxonomic and functional profiling of microbial communities
- AMR gene detection and resistome summaries
- Alpha/beta diversity analysis and rich data visualizations
- Cloud access, no local infrastructure needed
Learn when shotgun metagenomic sequencing can provide additional taxonomic and functional information compared with amplicon-based approaches.
KEPLER™ Host-Agnostic Microbiome Profiler
KEPLER™ Profiler provides host-agnostic, multi-kingdom taxonomic profiling across environmental, animal, agricultural, food, and other sample types using curated reference databases. Within Cosmos-Hub, KEPLER taxonomic results can be paired with host-agnostic functional profiling.
Benefits of KEPLER™ Profiler:
- Host-agnostic profiling across diverse sample types
- Multi-kingdom taxonomic profiling of bacteria, viruses, fungi, and protists
- Reference database encompassing more than 30,000 species
Figure 1: KEPLER™ Profiler provides host-agnostic, multi-kingdom metagenomic profiling with curated reference databases, 30,000+ species coverage, strain-level placement, fast run times, patented methods, and a literature base of 600+ citations.
CHAMP™ Human Microbiome Profiler
CHAMP™ Profiler provides high-resolution taxonomic and functional profiling of human-associated microbiomes from shotgun metagenomic sequencing data using a validated marker gene-based algorithm. It is designed for human microbiome, translational, and biotechnology research and supports analysis of microbial abundance and functional potential.
Benefits of CHAMP™ Profiler:
- Enhanced detection of human-associated microbes
- Species-level taxonomic profiling and functional potential analysis
Figure 2: CHAMP™ benchmark performance. In the CAMI benchmark, CHAMP showed 16% higher recall than MetaPhlAn4. In the NIBSC panel, CHAMP produced approximately 400-fold lower false signal than the other profilers shown.
Highly Efficient Ligation with Low Adapter Formation
The NEXTFLEX Rapid XP v3 DNA-Seq for Metagenomics workflow is designed to generate high-complexity libraries with low adapter-dimer formation and consistent representation across a broad GC range. These characteristics help preserve usable sequencing reads and support reliable analysis across diverse microbial genomes.
Compatible NEXTFLEX accessories
The NEXTFLEX™ Rapid XP v3 DNA-Seq Kit for Metagenomics pairs with NEXTFLEX UDI barcode adapters, sold separately, for sample multiplexing on Illumina® and Element Biosciences™ sequencing platforms. UDI configurations up to 1,536 indexes support projects ranging from small studies to high-throughput sequencing programs.
NEXTFLEX Normalization Beads are not included with the kit and are available separately.
Explore the NEXTFLEX NGS accessories portfolio to find the indexing or accessory format that best matches your workflow.
Specifications
| Analysis |
Metagenomics Analysis Included
|
|---|---|
| Automation Compatible |
Yes
|
| Product Group |
DNA-seq
|
| Shipping Conditions |
Dual Temperature
|
| Unit Size |
8 rxns
|
FAQs
-
What analysis is available through Cosmos-Hub?
-
Should I use CHAMP or KEPLER for my samples?
-
Which sequencing platforms are compatible?
Resources
Are you looking for resources, click on the resource type to explore further.
Loading...
How can we help you?
We are here to answer your questions.
