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AlphaLISA SureFire Ultra Multiplex Human Phospho-ALK (Tyr1604 & Total) Detection Kit, 100 Assay Points
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AlphaLISA SureFire Ultra Multiplex Human Phospho-ALK (Tyr1604 & Total) Detection Kit, 100 Assay Points
Alpha Terbium SureFire Ultra Multiplex
| Feature | Specification |
|---|---|
| Application | 細胞シグナル伝達 |
| Sample Volume | 30 µL |
Product variants
Unit Size: 500 assay points
Part #:
MPSU-PTALK-K500
Unit Size: 10,000 assay points
Part #:
MPSU-PTALK-K10K
Unit Size: 50,000 assay points
Part #:
MPSU-PTALK-K50K
Unit Size: 100 assay points
Part #:
MPSU-PTALK-K-HV
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Multiplex Human Phospho-ALK (Tyr1604 & Total) Detection Kit, 100 Assay Points
Alpha Terbium SureFire Ultra Multiplex
AlphaLISA SureFire Ultra Multiplex Human Phospho-ALK (Tyr1604 & Total) Detection Kit, 100 Assay Points
Product information
Overview
Anaplastic lymphoma kinase (ALK) also known as ALK tyrosine kinase receptor or CD246 (cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. ALK plays a pivotal role in cellular communication and in the normal development and function of the nervous system. The ALK gene can be oncogenic by forming a fusion gene with any of several other genes, by gaining additional gene copies or with mutations of the actual DNA code for the gene itself.
The AlphaLISA SureFire Ultra Multiplex Human Phospho-ALK (Tyr1604)/Total ALK Detection Kit is used to measure both phosphorylated and total levels of endogenous ALK in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated ALK analysis, and the 545 nm (Tb) signal corresponds to the total ALK analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
- No-wash steps, no separation steps
- Multiplexing assay
- Sensitive detection
- No interference from exogenous antibodies
- Results in less than 3 hours
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
How it works
Multiplex SureFire Ultra assay principle
The Multiplex SureFire Ultra assay simultaneously measures phospho and total protein at specific levels. It utilizes three antibodies that recognize the phospho epitope and two distal epitopes on the target protein. The assay requires three bead types: two distinct acceptor beads and one donor bead.
- The Alpha 615 acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody.
- The Alpha 545 acceptor bead is coated with the CaptSure2 antibody, which binds the CaptSure2-tagged anti-total target protein antibody.
- The Alpha donor bead binds the biotinylated anti-total target protein antibody.
When the phosphorylated protein is present, the three antibodies bring the donor and acceptor beads close together, allowing singlet oxygen to transfer energy and excite both acceptor beads. This generates a luminescent Alpha signal at 615nm and 545nm. The 615nm (Eu) signal is directly proportional to the amount of phosphorylated protein, while the 545nm (Tb) signal corresponds to the total protein levels.
Multiplex SureFire Ultra two-plate assay protocol
The two-plate protocol involves culturing and treating the cells in a 96-well plate before lysis, then transferring lysates into a 384-well Optiplate™ plate before the addition of Multiplex SureFire Ultra detection reagents. This protocol enables the cells viability and confluence to be monitored. In addition, lysates from a single well can be used to measure different targets.
Multiplex SureFire Ultra one-plate assay protocol
Detection of phosphorylated/total target proteins with Multiplex SureFire Ultra reagents can be performed in a single plate used for culturing, treatment, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust AlphaLISA SureFire Ultra quality.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra Multiplex
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
30 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
ALK
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Oncology
|
| Unit Size |
100 assay points
|
Video gallery
AlphaLISA SureFire Ultra Multiplex Human Phospho-ALK (Tyr1604 & Total) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Multiplex Human Phospho-ALK (Tyr1604 & Total) Detection Kit, 100 Assay Points
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Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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