| Feature | Specification |
|---|---|
| Application | 細胞シグナル伝達 |
| Sample Volume | 15 µL |
Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4014S.
Formats:
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
LANCE Ultra
|
| Cellular or Signaling Pathway |
TGFß/SMAD
|
| Detection Modality |
LANCE
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
15 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
SMAD3
|
| Target Class |
Phosphoproteins
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolic
|
| Unit Size |
10,000 Assay Points
|
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LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...
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