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LANCE Ultra Phospho-SMAD2 (Ser465/467) Detection Kit, 10,000 Assay Points
LANCE Ultra Phospho-SMAD2 (Ser465/467) Detection Kit, 10,000 Assay Points
LANCE Ultra Phospho-Protein
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 15 µL |
Product variants
Unit Size: 500 Assay Points
Part #:
TRF4012C
Unit Size: 10,000 Assay Points
Part #:
TRF4012M
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
LANCE Ultra Phospho-SMAD2 (Ser465/467) Detection Kit, 10,000 Assay Points
LANCE Ultra Phospho-Protein
LANCE Ultra Phospho-SMAD2 (Ser465/467) Detection Kit, 10,000 Assay Points
Product information
Overview
Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4012S.
Formats:
- Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
- Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
SMAD proteins are transcriptional activators activated by bone morphogenic proteins (BMPs). These ligands induce dimerization of SMAD proteins, whereby they translocate to the nucleus and bind target genes. SMAD1, 2, and 3 constitute receptor-regulated SMADs.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
LANCE Ultra
|
| Cellular or Signaling Pathway |
TGFß/SMAD
|
| Detection Modality |
LANCE
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
15 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
SMAD2
|
| Target Class |
Phosphoproteins
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolic
Oncology
Respiratory
|
| Unit Size |
10,000 Assay Points
|
Resources
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Product Info
LANCE and LANCE Ultra TR-FRET technology product list
LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...
Application Note
LANCE Ultra TR-FRET-based detection and modulation of phosphorylated STAT3 levels in human cells.
STAT3 is a particularly important therapeutic target since it is constitutively active in a range of cancer cells lines and human...
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