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LabChip High pI Charge Variant Analysis Kit
LabChip High pI Charge Variant Analysis Kit
Shipping box for Revvity reagent kits
| Feature | Specification |
|---|---|
| Instrument Compatibility | LabChip GXII Touch |
| Sample Type | Protein |
| Technology | Microfluidic Electrophoresis |
Product variants
タイプ: High pI (7.0 - 9.0)
Part #:
CLS760670
タイプ: Low pI (3.0 - 7.0)
Part #:
CLS154134
For research use only. Not for use in diagnostic procedures.
LabChip High pI Charge Variant Analysis Kit
Shipping box for Revvity reagent kits
LabChip High pI Charge Variant Analysis Kit
Product information
Overview
Increase the speed, efficiency, and reliability of charge variant analysis.
All biotherapeutic protein molecules come with charge heterogeneity sites, known as charge variants. Charge variants affect biological functions, such as binding and stability. Charge variant profiles also change dynamically throughout processes, such as sequence expression, chemical degradation, and post-translational modification. It is important to monitor and screen charge variant profiles of biotherapeutic proteins throughout the development and manufacturing processes.
Revvity’s LabChip® charge variant analysis assays are comparable to traditional CZE assays (Han, 2011) and can resolve and screen the charge variant profile of samples quickly, reliably, and automatically. With the throughput capability on the LabChip® GXII Touch™ protein characterization system, users can optimize their valuable research time to accelerate discovery and development of new biotherapeutic solutions.
Additional product information
Obtain speed and efficiency of charge variant analysis throughout biotherapeutic development and manufacturing processes.
- Simple and automated operational workflow
- Fast analysis speed: ~60 seconds per sample
- High throughput: 96 samples per experiment
- Minimal sample consumption: 25 μL
- Full pI range coverage: pI 3 – 9, both acidic and basic proteins
- Protein migration time can be adjusted based on running buffer pH
- Good linearity: peak intensity proportional to variant concentration
- Reliable repeatability with C.V. < 2.5 %
Figure 1. Charge variant profile of a positive charged mAb on the LabChip high pI charge variant assay. Due to a stronger electrophoretic force, basic variants move toward the cathode faster and arrive earlier at the detection window than main variants and acidic variants. Basic, main and acidic variants are separated, resolved and labeled in the LabChip charge variant assay. The relative concentration of each variant was also quantified.
Figure 2. LabChip Charge Variant Assays measure charge variant profile of protein reliably. Under pH =7.2 running buffer, charge variant profiles of bovine serum albumin exhibit same migration pattern. Migration time of main variant C.V. = 1.29% for two repeatable runs.
Ordering Information
The Charge Variant High PI Assay LabChip (CLS153419) is needed to run the assay. It is sold separately.
Specifications
| Application |
Protein Analysis
|
|---|---|
| Automation Compatible |
Yes
|
| Instrument Compatibility |
LabChip GXII Touch
|
| Sample Type |
Protein
|
| Shipping Conditions |
Dual Temperature
|
| Technology |
Microfluidic Electrophoresis
|
| Type |
High pI (7.0 - 9.0)
|
| Unit Size |
1 kit
|
References
- Han, H., Livingston, E., and Chen, X. (2011) High Throughput Profiling of Charge Heterogeneity in Antibodies by Microchip Electrophoresis Anal. Chem. 83, 21, 8184–8191. doi.org/10.1021/ac201741w.
Resources
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Product Note
Automated characterization of proteins and nucleic acids
Read the LabChip® GXII Touch™ protein characterization system product note to learn about an automated alternative to traditional...
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