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  • IVISense Fluorescent Dyes
  • IVISense 800 NHS Fluorescent Dye (5 mg) (VivoTag)

IVISense 800 NHS Fluorescent Dye (5 mg) (VivoTag)

IVISense 800
IVISense™ fluorescent imaging probes
IVISense 800 NHS Fluorescent Dye (5 mg) (VivoTag)
IVISense 800
IVISense™ fluorescent imaging probes
IVISense 800
IVISense™ fluorescent imaging probes
IVISense 800
IVISense 800
IVISense™ fluorescent imaging probes
IVISense 800
IVISense™ fluorescent imaging probes

Revvity’s IVISense™ 800 fluorescent dyes for labeling proteins, antibodies, peptides, and small molecules offer excellent brightness optimized for in vivo and in vitro imaging applications.

IVISense 800 fluorescent dyes are available as NHS esters for conjugation to free amine (-NH3) containing molecules.

View product information
Feature Specification
Wave Length 800 nm

Revvity’s IVISense™ 800 fluorescent dyes for labeling proteins, antibodies, peptides, and small molecules offer excellent brightness optimized for in vivo and in vitro imaging applications.

IVISense 800 fluorescent dyes are available as NHS esters for conjugation to free amine (-NH3) containing molecules.

View product information
Product variants
スタイル: NHS
Unit Size
1 mg
Part #:
NEV11107
スタイル: NHS
Unit Size
5 mg
Part #:
NEV11108
For research use only. Not for use in diagnostic procedures.
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IVISense 800
IVISense™ fluorescent imaging probes
IVISense 800
IVISense™ fluorescent imaging probes
IVISense 800 NHS Fluorescent Dye (5 mg) (VivoTag)
IVISense 800
IVISense™ fluorescent imaging probes
IVISense 800
IVISense™ fluorescent imaging probes
IVISense 800
IVISense 800 NHS Fluorescent Dye (5 mg) (VivoTag)
IVISense 800
IVISense™ fluorescent imaging probes
IVISense 800
IVISense™ fluorescent imaging probes
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Product information

  • Overview
  • Additional product information
  • Specifications

Overview

IVISense 800 NHS fluorescent dye is an amine-reactive near infrared (NIR) fluorochrome for coupling via an NHS ester linkage to peptides, small molecules, proteins, antibodies or macromolecules. Unit size 5 mg.

IVISense™ 800 fluorescent dyes are ideal for molecular labeling for use in preclinical in vivo imaging applications due to enhanced tissue penetration at this wavelength. IVISense 800 dyes are well suited for the labeling and imaging of proteins, antibodies, and small molecules in a wide range of research areas. The 800 nm fluorescence wavelength is ideal for biological imaging applications using in vivo fluorescence systems but will only work on microscopes, high content imaging and flow cytometry systems that have appropriate light sources and filter sets to accommodate this wavelength.

  • Uniquely optimized for the generation of in vivo imaging probes with low non-specific accumulation at tumor sites and inflammatory sites.
  • Need special microscopes, flow cytometers, and plate readers that accommodate excitation and emission with light sources and filters for this wavelength.
  • Suitable for in vivo multiplexing with 645 or 680 nm Revvity probes or other labeled proteins/Abs.  Expanded in vivo multiplexing with IVIS spectral unmixing with 645/680/750 nm probes or antibodies.
  • No spectral interference with mouse diets, so alfalfa-containing mouse diets can be used.
  • Recommended dye:protein ratio of 1-3.

Additional product information

Absorption and Emission Spectra
img-ivisense-800-nhs-dye-700x500px.jpg
Emission 810 nm +/- 5 nm
Absorbance 787 nm +/- 5 nm
IVIS Lumina series filter use (ex/em) 780/845 nm
IVIS Spectrum 2 series filter use (ex/em) 745/820 nm

 

Choose the right IVISense fluorescent dye for your study
Part Number Product Abs/Em (nm)  
IVISense NHS FLUORESCENT LABELING KIT
NEV11118 IVISense 680 NHS 665/688 NHS: Suitable for labeling free amines (typically lysine residues)

Each kit contains IVISense 680 NHS Fluorescent Dye and everything else needed to perform the labeling reaction and purify the labeled product. 
IVISense NHS FLUORESCENT DYES
NEV11173
NEV11174
IVISense 645 NHS 643/660 NHS: Suitable for labeling free amines (typically lysine residues)
NEV11119
NEV11120
IVISense 680 NHS 665/688
NEV11107
NEV11108
IVISense 800 NHS 785/810
IVISense MAL FLUORESCENT DYES
NEV11273
NEV11274
IVISense 645 MAL 643/660 MAL: Suitable for labeling free thiols (typically cysteine residues)
NEV11219
NEV11220
IVISense 680 MAL 665/688
IVISense SELF-QUENCHING DYES
NEV10121
NEV10122
IVISense 680 NHS 673/691 When a protein is labeled with multiple IVISense Self-Quenching Dye molecules, there is a shift in the absorbance of the dye, which will reduce the signal.

Only choose IVISense Self-Quenching Dyes if your labeling ratio of dye:protein will be 1:1. If multiple dyes are to be conjugated onto your protein, a non-self-quenching IVISense Dye should be used. 

NHS and MAL dyes are suitable as noted above.
NEV10123
NEV10124
IVISense 750 NHS 750/775
NEV11223
NEV11224
IVISense 750 MAL 750/776

 

Specifications

Brand
IVISense
Imaging Modality
Fluorescence
Shipping Conditions
Shipped in Blue Ice
Storage Conditions
-30 to -16 °C
Style
NHS
Unit Size
5 mg
Wave Length
800 nm

Resources

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Protocol/SOP
Protocol/SOP
PROTOCOL: Antibody and protein near infrared (NIR) labeling with IVISense 800 NHS fluorescent dye
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