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HTRF Testosterone Detection Kit, 500 Assay Points
HTRF Testosterone Detection Kit, 500 Assay Points
Generic HTRF biomarker competition XL665 image
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 10 µL |
Product variants
Unit Size: 500 assay points
Part #:
64TESPEG
Unit Size: 10,000 assay points
Part #:
64TESPEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Testosterone Detection Kit, 500 Assay Points
Generic HTRF biomarker competition XL665 image
HTRF Testosterone Detection Kit, 500 Assay Points
Product information
Overview
Testosterone is a steroid hormone synthesized from 17a-hydroxy progesterone by the enzymes 17,20-lyase and 17ß-hydroxysteroid dehydrogenase in the gonads of both males and females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid. It plays an important role in the stimulation and the development of masculine characteristics by its fixation of androgen receptors.
How it works
Assay principle
The testosterone assay is based on the competition principle, where native testosterone produced by cells and XL665-labelled testosterone compete for binding to a monoclonal anti-testosterone antibody labelled with Europium Cryptate.
Assay Protocol
The testosterone assay features a streamlined protocol with only two incubation steps: - Cell stimulation by target compounds - Testosterone detection using HTRF reagents This protocol requires a single one-hour incubation period following cell stimulation.
Assay details
Key features
| Detection limit: | 0.1 nM |
| Dynamic range: | 0.1 to 10 nM |
| EC50 | 0.84 nM |
| S/B | 13 |
| Specificity: | low cross-reactivity with 4-Androstene-3,17-Dione 1.3% |
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Biomarkers
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolism/Diabetes
Neuroscience
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Testosterone Detection Kit, 500 Assay Points
HTRF Testosterone Detection Kit, 500 Assay Points
Resources
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Brochure
HTRF assays and reagents product list
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
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