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HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
Shipping box for Revvity reagent kits
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Dynamic Range | 66 - 16,000 pg/mL |
| Limit of Detection | 15 pg/mL |
| Sample Volume | 16 µL |
Product variants
Unit Size: 500 assay points
Part #:
63ADK114PEG
Unit Size: 10,000 assay points
Part #:
63ADK114PEH
Unit Size: 50,000 assay points
Part #:
63ADK114PEY
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
Shipping box for Revvity reagent kits
HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
Product information
Overview
The SARS-CoV-2 Spike S1 protein is one of two part of the virus Spike protein (S). The S1 subunit is responsible for the the virus interaction with host cells' transmembrane Angiotensin-converting enzyme 2 receptors (ACE2), while the S2 subunit is capable of conformational changes that allow the fusion of the viral particle envelope and host cell membrane upon S1/ACE2 binding. As such, the S1 protein is closely investigated for its role in viral infection and is reliable marker of infection as well as a potential vaccine immunogen target.
How it works
SARS-CoV-2 particles are detected in cell lysates using a sandwich immunoassay involving an anti-S1 monoclonal antibody, labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the amount of S1 proteins.
The assay protocol, using a 384-well small volume plate or a low volume 96 well plate (20 µL final), is described on the right. 16 µL of cell lysates or control are dispensed directly into the detection plate for detection by HTRF reagents. The antibodies labelled with HTRF donor and acceptor can be pre-mixed and added in a single dispensing step to further streamline the assay procedure (4 µL), or separately (2 µL).
Assay details
Detection of recombinant SARS-CoV-2 S1 protein
16 µL of recombinant protein in lysis buffer #5 were dispensed into a low volume white microplate before the addition of 4 µL of the premixed HTRF anti SARS-CoV2 capsid detection reagents. The HTRF signal was recorded after ON incubation.
standard curve Spike S1 kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Time to result | ON at RT |
| Detection limit | 15 pg/mL |
| Dynamic range | 66 to 16,000 pg/mL |
| Sample compatibility | Cell-based or cell supernatant |
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Dynamic Range |
66 - 16,000 pg/mL
|
| Limit of Detection |
15 pg/mL
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Viral Particles
|
| Technology |
TR-FRET
|
| Unit Size |
10,000 assay points
|
Video gallery
HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
HTRF SARS-Cov2 Spike S1 protein Detection Kit, 10,000 Assay Points
Resources
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Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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