JP
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF Rabbit IgG-Fc Detection Kit, 500 Assay Points
HTRF Rabbit IgG-Fc Detection Kit, 500 Assay Points
Generic HTRF competitive antibody assay image
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 10 µL |
Product variants
Unit Size: 500 assay points
Part #:
6FRIGPEG
Unit Size: 10,000 assay points
Part #:
6FRIGPEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Rabbit IgG-Fc Detection Kit, 500 Assay Points
Generic HTRF competitive antibody assay image
HTRF Rabbit IgG-Fc Detection Kit, 500 Assay Points
Product information
Overview
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The Rabbit Fc kit enables a rapid quantification of rabbit IgG and any proteins containing a rabbit Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.
How it works
Assay principle
The rabbit Fc kit is a competitive immunoassay for the measurement of rIgGs or any rFc-tagged proteins in a range of biological materials. The assay uses an Eu3+-Cryptate conjugated antibody specific to rFc and XL665 coupled rIgGs. rFc contained in the sample competes with XL665-labelled rabbit IgG for binding to anti-rabbit Fc Eu Cryptate.
Assay Protocol
The rabbit Fc assay features a streamlined protocol with only 1 incubation step after sample and Rabbit Fc detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.
Assay details
Key features
| Rabbit Fc | |
|---|---|
| Detection limit: | 5 ng/mL |
| Dynamic range: | 4.9 80,000 ng/mL |
| Incubation time: | 2h at RT |
| EC50: | 178 ng/mL |
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Biologics
|
| Target Species |
Rabbit
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Rare Diseases
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Rabbit IgG-Fc Detection Kit, 500 Assay Points
HTRF Rabbit IgG-Fc Detection Kit, 500 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
Brochure
HTRF assays and reagents product list
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
How can we help you?
We are here to answer your questions.
