Revvity Sites Globally

Select your location.

*e-commerce not available for this region.

Australia

Austria

Belgium

Brazil *

Canada

China *

Denmark

Finland

France

Germany

Hong Kong (China) *

India *

Ireland

Italy

Japan *

Luxembourg

Mexico *

Netherlands

Norway

Philippines *

Republic of Korea *

Singapore *

Spain

Sweden

Switzerland

Thailand *

United Kingdom

United States

HTRF Prostaglandin E2 Detection Kit, 500 Assay Points

The Prostaglandin E2 kit is designed for the rapid detection of PGE2 in cell supernatant and whole cells.

View product information

Feature	Specification
Application	Protein Quantification
Dynamic Range	8 - 1,000 pg/mL
Limit of Detection	10 pg/mL
Sample Volume	10 µL

The Prostaglandin E2 kit is designed for the rapid detection of PGE2 in cell supernatant and whole cells.

View product information

Product variants

Unit Size: 500 assay points

Part #:

62P2APEG

Unit Size: 10,000 assay points

Part #:

62P2APEH

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

Request more information

Product information

Overview

Prostaglandin E2 (PGE2) is produced in multiple cell types from PGH2, a primary product of the arachidonic acid metabolism, via the prostaglandin synthase. PGE2 displays several biological functions, such as vasodilatation and smooth muscle relaxation, and is involved in pro- and anti-inflammation pathways. PGE2 production is a commonly used method for the detection of COX-1 and COX-2 modulation. The PGE2 kit is a highly sensitive method for quantifying PGE2, either in cell supernatant or directly in whole cells.

How it works

Assay principle

The PGE2 assay is based on the competition principle, where native PG2 produced by cells and d2-labelled PGE2 compete for binding to a monoclonal anti-PGE2 antibody labelled with Europium Cryptate. The assay can be run with several types of samples, such as cell supernatants or purified enzymes.

Assay Protocol

The PGE2 assay features a streamlined protocol with only 1 incubation step after sample and PGE2 detection reagents dispensing. This protocol requires a single 5-hour incubation period at RT.

Assay details

Key features

The PGE2 assay has a high level of flexibility. It can be performed using cell supernatants or directly on stimulated cells. The incubation time and temperature following addition of the detection reagents have little effect on the assay results, providing further assay flexibility.

Detection limit	10 pg/mL
Dynamic range	8 to 1000 pg/mL
EC50	250 pg/mL
S/B	31
Z'	0.9 for 20 µl assay volumes

Assay validation

PGE2 modulation upon Indomethacin inhibitor treatment

One benefit of the HTRF PGE2 assay is its ability to quantify PGE2 in the presence of cells without sacrificing assay performance. The results comparing direct cell-based versus supernatant transfer did not differ significantly. Monocytes were stimulated to produce PGE2 in the presence or absence of indomethacin, a known inhibitor of PGE2 production. The IC50 of indomethacin determined using HTRF technology (1.0 ± 0.4 nM) was in agreement with previously published data.

Specifications

Other specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Dynamic Range	8 - 1,000 pg/mL
Limit of Detection	10 pg/mL
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped Ambient
Target Class	Biomarkers
Technology	TR-FRET
Therapeutic Area	Cardiovascular Neuroscience Oncology & Inflammation
Unit Size	500 assay points