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HTRF Anti-p-Thr pAb Eu-Conjugate, 500 Assay Points
HTRF Anti-p-Thr pAb Eu-Conjugate, 500 Assay Points
Generic HTRF PAb EuK image
| Feature | Specification |
|---|---|
| Application | Biochemical Enzymatic Assay |
Product variants
Unit Size: 100,000 Assay Points
Part #:
61PTRKAY
Unit Size: 500 assay points
Part #:
61PTRKAE
Unit Size: 10,000 assay points
Part #:
61PTRKAZ
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Anti-p-Thr pAb Eu-Conjugate, 500 Assay Points
Generic HTRF PAb EuK image
HTRF Anti-p-Thr pAb Eu-Conjugate, 500 Assay Points
Product information
Overview
The Anti-phospho threonine-Eu cryptate is a labeled polyclonal antibody. It is highly specific for the presence of phosphothreonine in peptides or proteins and shows no cross-reactivity with the corresponding non-phosphorylated threonine residues. This Anti-Phosphothreonine antibody does not react with phosphotyrosine or phosphoserine. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.
How it works
Assay principle
HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
Assay protocol
HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Antiphosphothreonine-Eu cryptate prepared in a buffer containing EDTA to stop the enzymatic reaction.
Specifications
| Application |
Biochemical Enzymatic Assay
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Kinases
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Inflammation
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Anti-p-Thr pAb Eu-Conjugate, 500 Assay Points
HTRF Anti-p-Thr pAb Eu-Conjugate, 500 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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