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HTRF Anti-Phospho-MEK1/2 (Ser217/221) pAb Eu-Conjugate, 500 Assay Points
HTRF Anti-Phospho-MEK1/2 (Ser217/221) pAb Eu-Conjugate, 500 Assay Points
Generic HTRF PAb EuK image
PAb Anti-phospho MEK1/2-Eu cryptate enables the rapid detection of MEK1/2 phosphorylation on Ser 217/221 in a biochemical kinase assay format.
| Feature | Specification |
|---|---|
| Application | 生化学酵素アッセイ |
PAb Anti-phospho MEK1/2-Eu cryptate enables the rapid detection of MEK1/2 phosphorylation on Ser 217/221 in a biochemical kinase assay format.
Product variants
Unit Size: 100,000 Assay Points
Part #:
61P17KAY
Unit Size: 500 assay points
Part #:
61P17KAE
Unit Size: 10,000 assay points
Part #:
61P17KAZ
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Anti-Phospho-MEK1/2 (Ser217/221) pAb Eu-Conjugate, 500 Assay Points
Generic HTRF PAb EuK image
HTRF Anti-Phospho-MEK1/2 (Ser217/221) pAb Eu-Conjugate, 500 Assay Points
Product information
Overview
The Anti-phospho MEK1/2 substrate-Eu cryptate is a labeled polyclonal antibody enabling the detection of MEK1/2 when phosphorylated at Ser217/221. It recognizes singly phosphorylated MEK1/2 at Ser 217 but not MEK1/2 singly phosphorylated at Ser 221. It does not detect the non-phosphorylated MEK1/2. This allows the construction of a specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. HTRF allows to perform assay at physiological ATP concentrations.
HTRF assays offer many advantages over other technologies:
- Homogeneous add-and-read format
- No wash steps
- Low background
- Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
- Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
HTRF kinase assay use typically a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.
Assay protocol
HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho MEK1/2-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
Assay validation
Working with natural ligand, development of COT assay
As shown here, the HTRF Kinase toolbox reagents can be used with any tagged peptide or protein substrates. The performances of each format were compared to P33 filter-binding assay in the study published by J. Jia (Anal. Biochem. 2006:305:268-276 ). In format 1, anti-GST-XL665 and PAb Anti-phospho MEK1/2-Eu cryptate were used to detect the whole GST-tagged protein, while in format 2, a small biotinylated MEK1 peptide was detected using Streptavidin-XL665 and the same phospho specific labeled antibody. Results are published in the table below
Apparent ATP Km values determined by different COT assay formats
| Assay Format | Apparent ATP Km(µM) |
|---|---|
|
COT/MBP protein direct33P |
384 ± 55 |
|
COT/MEK protein direct HTRF |
279 ± 40 |
|
COT/MEK peptide direct HTRF |
268 ± 21 |
Specifications
| Application |
Biochemical Enzymatic Assay
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target |
MEK1/2
|
| Target Class |
Kinase
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Inflammation
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Anti-Phospho-MEK1/2 (Ser217/221) pAb Eu-Conjugate, 500 Assay Points
HTRF Anti-Phospho-MEK1/2 (Ser217/221) pAb Eu-Conjugate, 500 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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