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HTRF Myosin Heavy Chain Detection Kit, 500 Assay Points
HTRF Myosin Heavy Chain Detection Kit, 500 Assay Points
Generic HTRF biomarker image
| Feature | Specification |
|---|---|
| Application | タンパク質定量 |
| Sample Volume | 16 µL |
Product variants
Unit Size: 500 assay points
Part #:
63ADK098PEG
Unit Size: 10,000 assay points
Part #:
63ADK098PEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Myosin Heavy Chain Detection Kit, 500 Assay Points
Generic HTRF biomarker image
HTRF Myosin Heavy Chain Detection Kit, 500 Assay Points
Product information
Overview
Myosins are actin-based motor proteins that contribute to the generation of contractile force in eukaryotic cells. Each muscle myosin assembly contains a pair of myosin heavy chain (MYH) and two pairs of myosin light chain (MYL). MyHC-2 is a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This assay enables the detection of Myosin Heavy Chain in cell lysates for research related to skeletal muscle diseases.
How it works
Assay principle
Myosin Heavy Chain is measured using a sandwich immunoassay involving two specific anti-Myosin Heavy Chain antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the Myosin Heavy Chain present in the sample
Assay Protocol
The Myosin Heavy Chain assay protocol is described here. Cells are plated, stimulated, and lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of Myosin Heavy Chain. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Assay validation
Myosin heavy chain detection in C2C12 cells
C2C12 cells were grown in presence of different percentages of Fetal Bovine Serum (FBS) or Horse Serum (FS) to induce differentiation of C2C12 myoblasts into myotubes. After 4- or 8-day incubation, media was aspirated and cells were lysed with lysis buffer 1X for 30 min at RT under gentle shaking. 16µL of lysate were transferred into a 384-well sv white microplate and 4 µL of the Myosin Heavy Chain detection reagents were added. The HTRF signal was recorded after an overnight incubation at RT.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Biomarkers
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Rare Diseases
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Myosin Heavy Chain Detection Kit, 500 Assay Points
HTRF Myosin Heavy Chain Detection Kit, 500 Assay Points
Resources
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Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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