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HTRF Mouse IL-2 Detection Kit, 500 Assay Points

The HTRF mouse IL2 kit is designed for the quantification of mouse IL2 release in cell supernatant.

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Feature	Specification
Application	Protein Quantification
Sample Volume	16 µL

The HTRF mouse IL2 kit is designed for the quantification of mouse IL2 release in cell supernatant.

View product information

Product variants

Unit Size: 500 assay points

Part #:

62MIL02PEG

Unit Size: 10,000 assay points

Part #:

62MIL02PEH

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

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Product information

Overview

IL2's major role is to modulate cell-mediated immunity through its effect on T cells. IL2 promotes differentiation of T cells into effector or memory T cells, thus helping body fight off infection. IL2 is also a key player in immuno-oncology since it is main promoter of differentiation of T cells into Treg (regulatory T cells).

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.

How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting,
Revvity also worked with Myassays.com to help you in your data analysis., you’ll be able to access a free online software to run your IL2 analysis.

Assay details

Technical specifications of mouse IL2 kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	12 pg/mL &35 pg/mL
Range	35 2,560 pg/mL
Time to result	Overnight at RT
Species	Mouse only

Analytical performance

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [IL2] (pg/mL)	CV
1	100	9%
2	900	3%
3	1800	7%
	Mean CV	6%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[IL2] (pg/mL)	Mean (delta R)	CV
1	80	329	2%
2	320	1461	3%
3	1280	5980	8%
		Mean CV	4%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Specifications

Other specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	16 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Cytokines
Target Species	Mouse
Technology	TR-FRET
Therapeutic Area	Neuroscience Oncology & Inflammation
Unit Size	500 assay points