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HTRF Human MDA5 Aggregation Kit, 500 Assay Points
HTRF Human MDA5 Aggregation Kit, 500 Assay Points
Generic HTRF aggregated detection image
The MDA5 aggregation kit is designed for the fast detection of MDA5 aggregates in cell supernatants.
| Feature | Specification |
|---|---|
| Application | タンパク質定量 |
| Sample Volume | 16 µL |
The MDA5 aggregation kit is designed for the fast detection of MDA5 aggregates in cell supernatants.
Product variants
Unit Size: 500 assay points
Part #:
63ADK111PEG
Unit Size: 10,000 assay points
Part #:
63ADK111PEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human MDA5 Aggregation Kit, 500 Assay Points
Generic HTRF aggregated detection image
HTRF Human MDA5 Aggregation Kit, 500 Assay Points
Product information
Overview
MDA-5 is an RIG-I-like receptor that acts as a cytoplasmic sensor of double stranded RNA, and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines.
It is understood to form aggregates along RNA strands when performing its nucleic acid sensing functions.
It may also play an important role in enhancing natural killer cell function, and may be involved in growth inhibition and apoptosis in several tumor cell lines.
HTRF assays offer many advantages over other technologies:
- Homogeneous add-and-read format
- No wash steps
- Low background
- Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
- Stable signal, providing flexibility in time of readout or size of assays
How it works
MDA5 aggregation assay principle
MDA5 aggregates are measured using a sandwich immunoassay involving an anti-MDA5 monoclonal antibody specific for MDA5 aggregates, and labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the amount of MDA5 aggregates.
MDA5 aggregation assay protocol
The cell samples are transferred into the assay plate for the detection of MDA5 aggregates. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally
Assay validation
MDA5 aggregation in HUTU80 cells stimulated with Poly I;C
HUTU80 cells were plated in 96-well plates in complete culture medium, and incubated at 37°C, 5% CO2. The next day, cells were treated with Poly I:C (HMW)/LyoVec in low serum medium (0.5% FBS) for 2h at 37°C, 5% CO2. After treatment, cells were then lysed with supplemented lysis buffer #3 for 30 minutes at RT under gentle shaking, and 16 µL of lysate were transferred twice over into a low volume white microplate before the addition of 4 µL of the premixed HTRF aggregation MDA5 or Total-MDA5 detection reagents. The HTRF signal was recorded after ON incubation.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target |
MDA5
|
| Target Class |
Biomarkers
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Infectious Diseases
Inflammation
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Human MDA5 Aggregation Kit, 500 Assay Points
HTRF Human MDA5 Aggregation Kit, 500 Assay Points
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Brochure
HTRF assays and reagents product list
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
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