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HTRF LTB4 Detection Kit, 500 Assay Points
HTRF LTB4 Detection Kit, 500 Assay Points
Generic HTRF biomarker competition XL665 image
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 10 µL |
Product variants
Unit Size: 500 assay points
Part #:
64LB4PEG
Unit Size: 10,000 assay points
Part #:
64LB4PEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF LTB4 Detection Kit, 500 Assay Points
Generic HTRF biomarker competition XL665 image
HTRF LTB4 Detection Kit, 500 Assay Points
Product information
Overview
LTB4 is a major leukotriene product that is enzymatically derived from the arachidonic acid cascade. LTB4 is synthesized by leukocytes via the 5-lipoxygenase pathway and mediates different leukocyte functions such as lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been described as a potent mediator of inflammatory diseases and immunoregulation. The LTB4 kit enables the monitoring of LTB4 produced by cells and can be used to study the LTA4-hydrolase.
How it works
Assay principle
The LTB4 kit is a competitive immunoassay for the measurement of LTB4 in a range of biological formats. The assay uses an Eu3+-Cryptate conjugated antibody specific to LTB4 and XL665 coupled LTB4. LTB4 in the sample competes with XL665-labelled LTB4 for binding to anti-LTB4 Eu Cryptate.
Assay protocol
The LTB4 assay features a streamlined protocol with only 1 incubation step after sample and LTB4 detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.
Assay details
Key features
| LTB4 | |
| Incubation | 1h at RT |
| Assay range | 0.15 166.67 ng/mL |
| EC50 | 1.12 ng/mL |
| Detection limit | 0.15 ng/mL |
| Specificity | Low cross reactivity with 6-trans 12 epi LTB4 |
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Biomarkers
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Neuroscience
Oncology & Inflammation
|
| Unit Size |
500 assay points
|
Video gallery
HTRF LTB4 Detection Kit, 500 Assay Points
HTRF LTB4 Detection Kit, 500 Assay Points
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