JP
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF KRAS G12C / SOS1 Binding Kit Standard
HTRF KRAS G12C / SOS1 Binding Kit Standard
Generic Vial HTRF
| Feature | Specification |
|---|---|
| Application | タンパク質間相互作用 |
Product variant
Unit Size: 1 vial
Part #:
64KRASPPICDA
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF KRAS G12C / SOS1 Binding Kit Standard
Generic Vial HTRF
HTRF KRAS G12C / SOS1 Binding Kit Standard
Product information
Overview
KRAS/SOS1 binding standard can be used as a standard to control assay reproducibility. KRAS/SOS1 binding d is intended for use with the KRAS WT/SOS1 and the KRAS G12C/SOS1 kits.
How it works
Assay principle
The KRAS/SOS1 PPI assays include tagged human recombinant partners (KRAS WT or G12C and SOS1) and labelled anti-tag reagents for HTRF detection. Without any inhibitor, KRAS loaded with GTP binds to SOS1 , and the binding of each detection reagent to its tagged target generates an HTRF signal. In the presence of KRAS/SOS1 inhibitors or GTP competitors, the HTRF signal decreases.
Assay protocol
The Human KRAS/SOS1 binding assay can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, compounds or standards are dispensed directly into the assay plate. The pre-mixed GTP and human Tag1-KRAS protein is then added, together with the human Tag2-SOS1 protein, followed by the dispensing of the HTRF reagents, the anti-Tag2 antibody labeled with Terbium cryptate and the anti-Tag1 antibody labeled with XL665. The reagents labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step. No washing steps are needed. The protocol can be further miniaturized or upscaled by simply resizing each addition volume proportionally.
Assay validation
Standard curves with human KRAS WT and G12C
The KRAS/SOS1 binding standard inhibits the KRAS/SOS1 interaction for KRAS WT and KRAS G12C proteins in a correlated manner.
Specifications
| Application |
Protein-Protein Interaction
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Standard
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Binding Assay
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Oncology & Inflammation
|
| Unit Size |
1 vial
|
Video gallery
HTRF KRAS G12C / SOS1 Binding Kit Standard
HTRF KRAS G12C / SOS1 Binding Kit Standard
Resources
Are you looking for resources, click on the resource type to explore further.
Technical Note
How to access true Kd values in Protein-Protein Interaction assays
Investigate the dynamics of Interactions
Kinetic values are of importance for characterizing protein-protein interactions...
How can we help you?
We are here to answer your questions.
