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HTRF KinEASE TK Kit, 1,000 Assay Points

The HTRF KinEASE assay series is a semi-universal biochemical platform to study kinase activity. The TK kit uses a specific substrate compatible with tyrosine kinases - cf. validation data provided on this page.

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Feature	Specification
Application	Biochemical Enzymatic Assay
Sample Volume	10 µL

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Product variants

Unit Size: 1,000 assay points

Part #:

62TK0PEB

Unit Size: 20,000 assay points

Part #:

62TK0PEC

Unit Size: 100,000 assay points

Part #:

62TK0PEJ

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

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Product information

Overview

The HTRF KinEASE kit series provides a simple biochemical approach to study kinase activity, screen for inhibitors and characterize them. Three STK and one TK kits offer a semi-universal method for assessing phosphorylation on Serine/Threonine and Tyrosine residues, respectively. The HTRF KinEASE TK assay has been validated on 71 Tyrosine kinases, using a single generic substrate. The assay validation section of this page lists them and provides technical tips for setup.

How it works

Assay principle

Each HTRF KinEASE kit is based on the same assay format, and uses a universal biotinylated substrate, a phospho-specific Eu-cryptate-labeled monoclonal antibody, and SA-XL665. The TK generic substrate is made of a sequence of repetitive motifs recognized by most tyrosine kinases, and by the specific conjugated antibody once phosphorylated.

Assay protocol

As shown here, all HTRF KinEASE assays work the same way and involve two steps:

Step 1: Kinase reaction The kinase is incubated in the presence or absence of compounds and the appropriate substrate. The supplemented enzymatic buffer is added in the kinase buffer for tyr kinases only. ATP is added to start the reaction.

Step 2: Detection Eu-cryptate and XL665 conjugates are added. The detection buffer contains EDTA to stop the enzymatic reaction.

kinase-how-it-works-assay-principle-htrf-kinease

Assay validation

Today, the platform has been validated on the following kinases:

KinEASE TK	ABL1, ABL2, ALK, Arg, AXL, BLK, BMX, BRK, BTK, c-Kit, CSF1R, CSK, DDR2, EGFR, EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3, EPHB4, ERBB4, FAK, FER, FES, FGFR1, FGFR2, FGFR3, FGFR4, FGR, FLT1, FLT3, FLT4, FMS, Fps, FRK, FYN, HCK, HER4, IGF1R, INSR, INSRR, Insulin R, IRR, ITK, JAK1, JAK2, JAK3, KDR, KIT, LCK, Lyn, LYNa, MER, MERTK, MET, MST1R, MSTIR, MUSK, NTRK1, NTRK2, NTRK3, PDGFR α, PDGFR β, PDGFRA, PDGFRB, PTK2, PTK2B, PTK5, PTK6, PYK2, RET, RON, ROS, ROS1, Rse, SRC, SYK, TEK, TIE2, TRKA, TRKB, TRKC, TYK2, TYR03, TYRO3, VEGFR1, VEGFR2, VEGFR3, YES1, ZAP70

KinEASE TK

ABL1, ABL2, ALK, Arg, AXL, BLK, BMX, BRK, BTK, c-Kit, CSF1R, CSK, DDR2, EGFR, EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3, EPHB4, ERBB4, FAK, FER, FES, FGFR1, FGFR2, FGFR3, FGFR4, FGR, FLT1, FLT3, FLT4, FMS, Fps, FRK, FYN, HCK, HER4, IGF1R, INSR, INSRR, Insulin R, IRR, ITK, JAK1, JAK2, JAK3, KDR, KIT, LCK, Lyn, LYNa, MER, MERTK, MET, MST1R, MSTIR, MUSK, NTRK1, NTRK2, NTRK3, PDGFR α, PDGFR β, PDGFRA, PDGFRB, PTK2, PTK2B, PTK5, PTK6, PYK2, RET, RON, ROS, ROS1, Rse, SRC, SYK, TEK, TIE2, TRKA, TRKB, TRKC, TYK2, TYR03, TYRO3, VEGFR1, VEGFR2, VEGFR3, YES1, ZAP70

Specifications

Other specifications

Application	Biochemical Enzymatic Assay
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped Ambient
Target Class	Kinases
Technology	TR-FRET
Therapeutic Area	Metabolism/Diabetes NASH/Fibrosis Oncology & Inflammation
Unit Size	1,000 assay points