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HTRF Insulin Ultra Sensitive Detection Kit, 500 Assay Points
HTRF Insulin Ultra Sensitive Detection Kit, 500 Assay Points
Generic HTRF biomarker image
| Feature | Specification |
|---|---|
| Application | タンパク質定量 |
| Sample Volume | 5 µL |
Product variants
Unit Size: 500 assay points
Part #:
62IN2PEG
Unit Size: 10,000 assay points
Part #:
62IN2PEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Insulin Ultra Sensitive Detection Kit, 500 Assay Points
Generic HTRF biomarker image
HTRF Insulin Ultra Sensitive Detection Kit, 500 Assay Points
Product information
Overview
One hurdle faced by researchers seeking to quantify insulin is the high number of islets often required per assay. Designed for enhanced sensitivity, this kit is intended for the quantification of insulin in low-concentration samples. The kit is ideal for the detection of basal insulin levels and insulin released from mouse pancreatic islets.
How it works
Assay principle
Insulin is measured using a sandwich immunoassay of two monoclonal antibodies, one labelled with Cryptate (donor) and the other with XL665 (acceptor). The intensity of the FRET signal obtained is proportional to the concentration of insulin in the sample.
Assay protocol
10 µL of cell supernatants are supplemented with 5 µL of acceptor-labelled antibodies and 5 µL of donor-labelled antibodies (or 10 µL of pre-mixed reagents for a single dispensing step). The mix is then incubated from 4h to overnight at RT and results are read on an HTRF compatible reader.
Assay details
Correlation with leading RIA
The HTRF Ultra Sensitive insulin assay showed an excellent correlation to the leading RIA on mouse pancreatic islet supernatants. With a slope of 0.946, the regression line was close to the ideal line, demonstrating the assay's reliability.
Assay validation
Validation on mouse pancreatic islets
Pancreatic islets were isolated from C57BL/6N male mice. The islets were dispensed into tubes with KRB buffer containing either 2.8 mM or 16.7 mM glucose (5 islets/tube, 6 tubes/condition). After 10 minutes or 30 minutes of incubation, the supernatants* were collected and the insulin concentrations measured using the HTRF Ultra-sensitive insulin kit reagents.
*Samples were kindly provided by S. Dalle and M. Ravier’s research team: Physiopathology of pancreatic beta-cells, Institute for Functional Genomics (IGF), Montpellier, France.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
5 µL
|
| Shipping Conditions |
Shipped Ambient
|
| Target Class |
Biomarkers
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolism/Diabetes
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Insulin Ultra Sensitive Detection Kit, 500 Assay Points
HTRF Insulin Ultra Sensitive Detection Kit, 500 Assay Points
Citations
Resources
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Flyer
Obesity, diabetes and metabolic disease reagents
This flyer details HTRF™ and AlphaLISA™ assays for investigating key biomarkers and signaling pathways in metabolic disease...
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