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HTRF Human Hyaluronic acid Detection Kit, 500 Assay Points
HTRF Human Hyaluronic acid Detection Kit, 500 Assay Points
Generic HTRF Hyaluronic acid image
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 5 µL |
Product variant
Unit Size: 500 assay points
Part #:
63ADK089PEG
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human Hyaluronic acid Detection Kit, 500 Assay Points
Generic HTRF Hyaluronic acid image
HTRF Human Hyaluronic acid Detection Kit, 500 Assay Points
Product information
Overview
Hyaluronic acid (HA), also called hyaluronan, is an important component of the extracellular matrix contributing significantly to cell proliferation and migration. HA may also be involved in the progression of some malignant tumors. HA acts as a promoter of early inflammation, which is crucial to the whole skin wound-healing process. The hyaluronic acid kit is designed for a rapid detection of HA in cell supernatants.
How it works
Assay principle
Hyaluronic Acid is measured using a sandwich immunoassay involving Hyaluronic Acid Binding Protein (HABP) labelled with terbium Cryptate (donor) and HABP labelled with d2 (acceptor). The intensity of the signal is proportional to the concentration of Hyaluronic Acid present in the sample.
Assay Protocol
Cells are plated and stimulated in the same 96-well culture plate. Cell supernatants are then transferred into the assay plate for the detection of Hyaluronic Acid by HTRF reagents. This protocol enables the cells' viability and confluence to be monitored. The binding buffer is required to enhance the detection of Hyaluronic Acid on samples. The HABP labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
5 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Biomarkers
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolism/Diabetes
NASH/Fibrosis
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Human Hyaluronic acid Detection Kit, 500 Assay Points
HTRF Human Hyaluronic acid Detection Kit, 500 Assay Points
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