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HTRF Human IL-22 Detection Kit, 10,000 Assay Points
HTRF Human IL-22 Detection Kit, 10,000 Assay Points
Generic HTRF cytokine image
| Feature | Specification |
|---|---|
| Application | タンパク質定量 |
| Sample Volume | 16 µL |
Product variants
Unit Size: 500 assay points
Part #:
62HIL22PEG
Unit Size: 10,000 assay points
Part #:
62HIL22PEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human IL-22 Detection Kit, 10,000 Assay Points
Generic HTRF cytokine image
HTRF Human IL-22 Detection Kit, 10,000 Assay Points
Product information
Overview
Interleukin 22 is a type 2 cytokine (member of the IL10 family) and plays an essential role in innate protective immunity against extracellular pathogens. It induces pro-inflammatory responses, allows production of antimicrobial peptides. IL-22 is also involved in wound-healing and regeneration of epithelial cells. Human IL-22 is produced on inflammation site by Th17 CD4+ cells, activated T-cells, Th1 and NK cells. After being secreted, it targets non-hematopoietic cells and binds to IL22R that is restricted to tissue-resident cells (fibroblasts, epithelial cells). Downstream signaling involves phosphorylation of Jak1 and Tyk2 and subsequent activation of STAT3. Cisbio’s Human IL22 kit is a homogeneous, add and read assay to measure IL-22 secretion in cell supernatants. It offers a fast, efficient alternative to ELISA.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
How it works
Assay Principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µL). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing, and also 4PL 1/y² fitting.
Revvity also works with Myassays.com to help you in your data analysis. You’ll be able to access a free online software to run your IL22 analysis.
Assay details
Technical specifications of human IL22 kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD & LOQ (in Diluent) | 9 pg/mL & 26 pg/mL |
| Range | 26 – 5,000 pg/mL |
| Time to result | Overnight at RT (reading possible at 3h) |
| Calibration | No international standard available |
| Species | Human only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IL22] (pg/mL) | CV |
|---|---|---|
| 1 | 78 | 8% |
| 2 | 411 | 5% |
| 3 | 5000 | 6% |
| Mean CV | 6.3% |
Each of the 3 samples was measured 24 times, and %CV for concentration was calculated for each sample.
Inter-assay (n=4)
| Sample | [IL22] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 78 | 471 |
9.4% |
| 2 | 411 | 2104 |
3.1% |
| 3 | 5000 | 15181 |
1.7% |
| Mean CV | 4.7 |
Each of the samples was measured in 4 different experiments, and %CV for concentration was calculated for each sample.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Cytokines
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Inflammation
|
| Unit Size |
10,000 assay points
|
Video gallery
HTRF Human IL-22 Detection Kit, 10,000 Assay Points
HTRF Human IL-22 Detection Kit, 10,000 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
Flyer
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