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HTRF Human IL-17 Detection Kit, 96 Assay Points
HTRF Human IL-17 Detection Kit, 96 Assay Points
Generic HTRF cytokine image
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 16 µL |
Product variants
Unit Size: 96 assay points
Part #:
62HIL17PET
Unit Size: 500 assay points
Part #:
62HIL17PEG
Unit Size: 10,000 assay points
Part #:
62HIL17PEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human IL-17 Detection Kit, 96 Assay Points
Generic HTRF cytokine image
HTRF Human IL-17 Detection Kit, 96 Assay Points
Product information
Overview
IL17 is a pro-inflammatory cytokine induced by IL23 and produced by helper T cells. IL17 is part of response to fungi and bacteria, and promotes release of or pro-inflammatory cytokines and chemokines. Due to its role as a mediator of inflammatory responses, IL17 has been linked to allergic responses and autoimmune diseases like asthma, psoriasis, or IBD (inflammatory bowel disease). In immuno-oncology, IL17 is main cytokine released by differentiated TH17 cells.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
How it works
Assay Principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page. Revvity also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL17 analysis.
Assay details
Technical specifications of human IL17 kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers &protocol. |
| LOD &LOQ (in Diluent) | 7 pg/mL &33 pg/mL |
| Range | 33 10,000 pg/mL |
| Time to result | Overnight at RT |
| Calibration | NIBSC (01/420) value (IU/mL) = 0,02 x HTRF hIL17 value (pg/mL) |
| Species | Human only |
Analytical performance
Intra and inter assay
Intra-assay (n=24)
| Sample | Mean [IL17] (pg/mL) | CV |
|---|---|---|
| 1 | 114 | 6% |
| 2 | 4644 | 2% |
| 3 | 8634 | 2% |
| Mean CV | 3% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter-assay (n=4)
| Sample | [IL17] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 194 | 350 | 6% |
| 2 | 939 | 1848 | 11% |
| 3 | 4545 | 8485 | 3% |
| Mean CV | 7% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Cytokines
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
NASH/Fibrosis
Oncology & Inflammation
|
| Unit Size |
96 assay points
|
Video gallery
HTRF Human IL-17 Detection Kit, 96 Assay Points
HTRF Human IL-17 Detection Kit, 96 Assay Points
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