JP
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF Human IgG Detection Kit, 500 Assay Points
HTRF Human IgG Detection Kit, 500 Assay Points
Generic HTRF IgG sandwich image
| Feature | Specification |
|---|---|
| Application | タンパク質定量 |
| Sample Volume | 10 µL |
Product variants
Unit Size: 500 assay points
Part #:
6FHIGPEG
Unit Size: 10,000 assay points
Part #:
6FHIGPEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human IgG Detection Kit, 500 Assay Points
Generic HTRF IgG sandwich image
HTRF Human IgG Detection Kit, 500 Assay Points
Product information
Overview
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The human IgG kit enables a rapid quantification of human IgG in cell supernatant and the selection of the best producer clones. The assay is compatible with natural IgG as well as recombinant antibodies.
How it works
Assay principle
This kit is a sandwich immunoassay enabling highly sensitive quantification of human IgG, even when using a small sample volume. The assay uses two polyclonal antibodies, one (Fc specific) labelled with Cryptate and the second (FAb specific) with d2. The signal intensity is proportional to the number of antibody-antibody complexes formed and therefore to the human IgG concentration.
Assay Protocol
The human IgG assay features a streamlined protocol with only 1 incubation step after sample and human IgG detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.
Assay details
Key features
The figure below shows the human IgG kit calibration curve. The assay was performed in a 384-well small volume white microplate in a 20 µL total volume, using 10µL human IgG calibrator diluted in PBS 0.1 M and 5 µL of each conjugate. The two HTRF conjugates may be pre-mixed and added in a single dispensing step to further streamline the protocol. After 2h of incubation, the signal was read on a PHERAstarFS flash lamp.
| Detection limit | 0.9 ng/mL |
| Assay range | 0.9 to 2000 ng/mL |
| Incubation time | 2h at RT |
| Specificity | IgG1, IgG2, IgG3, IgG4 |
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Biologics
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Rare Diseases
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Human IgG Detection Kit, 500 Assay Points
HTRF Human IgG Detection Kit, 500 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
Brochure
HTRF assays and reagents product list
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
How can we help you?
We are here to answer your questions.
