JP
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF Human GM-CSF Detection Kit, 10,000 Assay Points
HTRF Human GM-CSF Detection Kit, 10,000 Assay Points
Generic HTRF biomarker image
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 16 µL |
Product variants
Unit Size: 500 assay points
Part #:
62HGMCSFPEG
Unit Size: 10,000 assay points
Part #:
62HGMCSFPEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human GM-CSF Detection Kit, 10,000 Assay Points
Generic HTRF biomarker image
HTRF Human GM-CSF Detection Kit, 10,000 Assay Points
Product information
Overview
Also known as CSF2 (colony-stimulating factor 2), GM-CSF (granulocyte-macrophage colony-stimulating factor) is a hematopoietic growth factor and an immune modulator. Under immune stimuli, it is produced by T lymphocytes, macrophages, endolial cells, and fibroblasts. It acts on precursor cells in bone marrow to initiate ir proliferation and differentiation into mature granulocytes and macrophages. GM-CSF stimulates multipotent progenitor cells.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Revvity also worked with myassays.com to help you in your data analysis.
Assay details
Technical specifications of human GM-CSF kit
| Sample size | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers & protocol. |
| LOD & LOQ (in Diluent) | 27 pg/mL & 47 pg/mL |
| Range | 47 – 4,500 pg/mL |
| Time to result | Overnight at RT |
| Calibration | NIBSC (09/136) value (IU/mL) = 0,07 x HTRF hGCSF HTRF |
| Species | Human only |
Analytical performance
Intra and inter assay
Intra assay (n=24)
| Sample | Mean [GM-CSF] (pg/mL) | CV |
|---|---|---|
| 1 | 81 | 17% |
| 2 | 2125 | 4% |
| 3 | 6011 | 10% |
| Mean CV | 10% |
Inter assay (n=4)
| Sample | [GM-CSF] (pg/mL) | Mean (delta ratio) | |
|---|---|---|---|
| 1 | 169 | 370 | 6% |
| 2 | 774 | 1654 | 7% |
| 3 | 4135 | 5313 | 6% |
| Mean CV | 7% |
Assay validation
GM-CSF secretion by PBMC co-stimulated with ionomycin and PMA
PBMCs, plated at 100,000 cells/well in a 96 well plate, were stimulated for 24h with 1µg/ml of ionomycin and with various concentrations of PMA. 16µl of supernatant were transferred into a 384 well (small volume) white detection plate to be analyzed by the Human GM-CSF Assay.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Cytokines
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Oncology & Inflammation
|
| Unit Size |
10,000 assay points
|
Video gallery
HTRF Human GM-CSF Detection Kit, 10,000 Assay Points
HTRF Human GM-CSF Detection Kit, 10,000 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
Flyer
Reagent solutions for autoimmunity research.
Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies
How can we help you?
We are here to answer your questions.
