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HTRF Human G-CSF Detection Kit, 500 Assay Points

The HTRF human G-CSF kit is designed for the quantification of human G-CSF release in cell supernatant.

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Feature	Specification
Application	Protein Quantification
Sample Volume	16 µL

The HTRF human G-CSF kit is designed for the quantification of human G-CSF release in cell supernatant.

View product information

Product variants

Unit Size: 500 assay points

Part #:

62HGCSFPEG

Unit Size: 10,000 assay points

Part #:

62HGCSFPEH

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

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Product information

Overview

Also known as CSF3 (colony-stimulating factor 3), G-CSF is a hematopoietic cytokine. It acts on precursor cells in bone marrow to initiate ir proliferation and differentiation into mature granulocytes, on hematopoietic stem cells to induce ir mobilization in blood stream, and on neurons to promote neurogenesis and block apoptosis. In myocardial infarction or ischemia, G-CSF promotes T cells' immune tolerance and tissue recovery. absence of a functional G-CSF receptor is associated with congenital neutropenia.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.

How it works

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases. To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting. Revvity also worked with Myassays.com to help you in your data analysis.

Assay details

Technical specifications of human G-CSF kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	27 pg/mL &47 pg/mL
Range	47 4,500 pg/mL
Time to result	Overnight at RT
Calibration	NIBSC (09/136) value (IU/mL) = 0,07 x HTRF hGCSF HTRF
Species	Human only

Analytical performance

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [G-CSF] (pg/mL)	CV
1	47	18%
2	2036	7%
3	3780	6%
	Mean CV	10%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[G-CSF] (pg/mL)	Mean (delta R)	CV
1	87	222	13%
2	423	883	8%
3	2045	3161	6%
		Mean CV	9%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Specifications

Other specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	16 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Cytokines
Target Species	Human
Technology	TR-FRET
Therapeutic Area	Cardiovascular Neuroscience Oncology & Inflammation
Unit Size	500 assay points