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HTRF Human CCL2 Detection Kit, 10,000 Assay Points
HTRF Human CCL2 Detection Kit, 10,000 Assay Points
Generic HTRF biomarker image
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 16 µL |
Product variants
Unit Size: 500 assay points
Part #:
62HCCL2PEG
Unit Size: 10,000 assay points
Part #:
62HCCL2PEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human CCL2 Detection Kit, 10,000 Assay Points
Generic HTRF biomarker image
HTRF Human CCL2 Detection Kit, 10,000 Assay Points
Product information
Overview
Also called MCP-1 (monocyte chemoattractant protein 1), CCL2 is a chemokine mainly secreted by monocytes, macrophages, and dendritic cells to attract monocytes, basophils, and T cells. CCL2 is involved in pathologies like psoriasis or arosclerosis. In brain, CCL2 is released by neurons, astrocytes, and microglia, and is part of neuro-inflammatory response observed in ischemia or Alzheimer's disease. CCL2 is also secreted by adipocytes and can interfere with insulin signaling, thus suggesting a role in inflammation linked to diabetes.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma.
How it works
Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.
Assay data analysis
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
Revvity also worked with Myassays.com to help you in your data analysis.
Assay details
Technical specifications of human CCL2 kit
| Sample | 16 µL |
|---|---|
| Final assay volume | 20 µL |
| Kit components | Lyophilized standard, frozen detection antibodies, buffers & protocol. |
| LOD & LOQ (in Diluent) | 20 pg/mL & 90 pg/mL |
| Range | 90 - 3,000pg/mL |
| Time to result | ON at RT |
| Calibration | NIBSC (92/794) value (IU/mL) = 0,9 x HTRF hCCL2 value (pg/mL) |
| LOD & LOQ (in Diluent) | 20 pg/mL & 90 pg/mL |
| Species | Human only |
Analytical performance
Intra and inter assay
| Sample | Mean [CCL2] (pg/mL) | CV |
|---|---|---|
| 1 | 100 | 10% |
| 2 | 1500 | 7% |
| 3 | 2750 | 7% |
| 8% |
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
Inter assay (n=4)
| Sample | [CCL2] (pg/mL) | Mean (delta R) | CV |
|---|---|---|---|
| 1 | 121 | 335 | 2% |
| 2 | 437 | 1096 | 3% |
| 3 | 1579 | 3030 | 2% |
| Mean CV | 2% |
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Cytokines
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolism/Diabetes
NASH/Fibrosis
Oncology & Inflammation
|
| Unit Size |
10,000 assay points
|
Video gallery
HTRF Human CCL2 Detection Kit, 10,000 Assay Points
HTRF Human CCL2 Detection Kit, 10,000 Assay Points
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