JP
Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF Human ApoA1 Detection Kit, 500 Assay Points
HTRF Human ApoA1 Detection Kit, 500 Assay Points
Generic HTRF biomarker competition XL665 image
This product has been discontinued
The ApoA1 kit is designed for the simple quantification of ApoA1 in cell-based formats.
| Feature | Specification |
|---|---|
| Application | タンパク質定量 |
| Sample Volume | 5 µL |
The ApoA1 kit is designed for the simple quantification of ApoA1 in cell-based formats.
Product variants
Discontinued
Unit Size: 500 assay points
Part #:
64APAPEG
Discontinued
Unit Size: 10,000 assay points
Part #:
64APAPEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human ApoA1 Detection Kit, 500 Assay Points
Generic HTRF biomarker competition XL665 image
HTRF Human ApoA1 Detection Kit, 500 Assay Points
Product information
Overview
Apolipoproteins are proteins that bind to lipids to form lipoproteins, whose main function is to transport lipids. Apolipoproteins are important in maintaining the structural integrity and solubility of lipoproteins, and play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. ApoA1 is the major protein component of high-density lipoprotein (HDL). Deficiency of ApoA1 is associated with HDL deficiencies, including systemic non-neuropathic amyloidosis. ApoA1 may also have a role in protection against Alzheimer's disease.
HTRF assays offer many advantages over other technologies:
- Homogeneous add-and-read format
- No wash steps
- Low background
- Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
- Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
The Apolipoprotein A1 assay is a sandwich immunoassay involving two monoclonal antibodies, one labelled with Eu-Cryptate (Donor) and the second with d2 (Acceptor). The intensity of the signal is proportional to the concentration of apolipoprotein present in the sample.
Assay protocol
The ApoA1 assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires a single 3-hour incubation period at RT.
Assay details
Key features
| Cross-reactivity (%) | ApoA1 |
|---|---|
| ApoA1 | 100 |
| ApoB-100 | 0.044 |
| ApoA2 | 0.037 |
| ApoC1 | 0.014 |
| ApoC2 | 0.004 |
| ApoC3 | 0.005 |
| ApoE | 0.004 |
| ApoB-48 | ND |
Assay validation
ApoA1 detection upon retinoid X receptor (RXR) agonist treatment
Human hepatoma cells (HepG2) in 80-90% confluence were grown in DMEM (Gibco; 11885, low glucose) supplemented with 2% FBS. 5 µL of HepG2 cells (2,000 & 5,000 per well) and 5 µL of the RXR ligand 9-cis retinoic acid (9-cis-RA) in DMEM, 2% FBS at various concentrations were dispensed into a 384-well low volume black culture plate. After a 48 hour incubation at 37°C in CO2 atmosphere, 10 µL of the two pre-mixed detection reagents were added.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
5 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Biomarkers
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Human ApoA1 Detection Kit, 500 Assay Points
HTRF Human ApoA1 Detection Kit, 500 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
Brochure
HTRF assays and reagents product list
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
How can we help you?
We are here to answer your questions.
