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HTRF Human Pro-Collagen Type 1 Detection Kit, 10,000 Assay Points
HTRF Human Pro-Collagen Type 1 Detection Kit, 10,000 Assay Points
Generic HTRF biomarker image
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 16 µL |
Product variants
Unit Size: 500 assay points
Part #:
63ADK014PEG
Unit Size: 10,000 assay points
Part #:
63ADK014PEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human Pro-Collagen Type 1 Detection Kit, 10,000 Assay Points
Generic HTRF biomarker image
HTRF Human Pro-Collagen Type 1 Detection Kit, 10,000 Assay Points
Product information
Overview
Human pro-collagen type 1 is made by a combined association of two pro-alpha1 chains with a pro-alpha2 chain. These triple-stranded, rope-like pro-collagen molecules must be processed by enzymes outside the cell to form fibrils that cross-link to produce strong mature type I collagen fibers. The human pro-collagen type 1 kit is designed for the quantitative detection of human pro-collagen type 1, using cell supernatants, whole cells, or recombinant protein extracts.
How it works
Assay principle
Human pro-collagen is measured using a sandwich immunoassay involving two specific anti-human pro-collagen antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of pro-collagen present in the sample.
Assay protocol
The human pro-collagen type 1 assay protocol is shown here using a 384-well small volume white plate (20 µL final). Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection of human pro-collagen by HTRF reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Biomarkers
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
NASH/Fibrosis
Rare Diseases
|
| Unit Size |
10,000 assay points
|
Video gallery
HTRF Human Pro-Collagen Type 1 Detection Kit, 10,000 Assay Points
HTRF Human Pro-Collagen Type 1 Detection Kit, 10,000 Assay Points
Citations
Resources
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Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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