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HTRF Human Amyloid-β 1-40 Detection Kit, 500 Assay Points
HTRF Human Amyloid-β 1-40 Detection Kit, 500 Assay Points
Generic HTRF biomarker image
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 5 µL |
Product variants
Unit Size: 500 assay points
Part #:
62B40PEG
Unit Size: 10,000 assay points
Part #:
62B40PEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human Amyloid-β 1-40 Detection Kit, 500 Assay Points
Generic HTRF biomarker image
HTRF Human Amyloid-β 1-40 Detection Kit, 500 Assay Points
Product information
Overview
Amyloid ß1-40 and ß1-42 peptides are involved in the appearance of Alzheimer's disease symptoms. The production of these two amyloid peptides is regulated by the activity of 2 enzymes, ß and γ secretase, which cleave the amyloid precursor protein. This kit is intended to measure amyloid ß1-40 peptides in cell supernatant or in whole cells. This assay is a fast alternative to ELISA, thanks to the ease-of-use of our Add and Read method.
How it works
Assay principle
The HTRF Amyloid ß1-40 assay is a sandwich immunoassay using two monoclonal antibodies that recognize different epitopes within the ß1-40 peptide. One antibody is labelled with Eu3+-Cryptate and the other with XL-665. The assay can be used with whole cells or cell supernatant.
Assay protocol
The Amyloid ß1-40 assay features a streamlined protocol with only two incubation steps:
- Cell stimulation by target compounds
- Amyloid ß1-40 detection using HTRF reagents
The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further speed up the assay procedure. This protocol requires a single 24-hour incubation at 4°C following cell stimulation.
Assay details
Key features
| Detection limit: | 13.64 pg/mL |
|---|---|
| Dynamic range: | 25 to 1600 pg/mL |
| Sample compatibility: | Cell-based or cell supernatants |
| Specificity: | No cross-reaction with Amyloid ß 1-42 and ß 1-43 |
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
5 µL
|
| Shipping Conditions |
Shipped Ambient
|
| Target Class |
Biomarkers
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Neuroscience
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Human Amyloid-β 1-40 Detection Kit, 500 Assay Points
HTRF Human Amyloid-β 1-40 Detection Kit, 500 Assay Points
Citations
Resources
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