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HTRF Human HSA Detection Kit, 500 Assay Points
HTRF Human HSA Detection Kit, 500 Assay Points
Generic HTRF biomarker image
The HSA kit is designed for the rapid detection of HSA in cell supernatant.
| Feature | Specification |
|---|---|
| Application | タンパク質定量 |
| Sample Volume | 10 µL |
The HSA kit is designed for the rapid detection of HSA in cell supernatant.
Product variants
Unit Size: 500 assay points
Part #:
6FHSAPEG
Unit Size: 10,000 assay points
Part #:
6FHSAPEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human HSA Detection Kit, 500 Assay Points
Generic HTRF biomarker image
HTRF Human HSA Detection Kit, 500 Assay Points
Product information
Overview
Human Serum Albumin (HSA) is a protein produced in the liver that represents the most abundant component in human blood plasma. Serum Albumin's main function is the regulation of the colloidal osmotic pressure of blood. The kit is intended for the specific and fast quantification of HSA in cell supernatants.
HTRF assays offer many advantages over other technologies:
- Homogeneous add-and-read format
- No wash steps
- Low background
- Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
- Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
The HSA assay is a sandwich immunoassay. The assay uses two specific anti-human serum albumin antibodies, one labelled with Eu3+-Cryptate and the other with d2. The specific signal is proportional to the concentration of HSA present in the sample. This homogeneous assay requires only a single reagent addition (by premixing detecting reagents) and an ON incubation for the quantitative detection of CHO HCP.
Assay Protocol
The HSA assay features a streamlined protocol with only one incubation step following the sample/calibrator and Antibody conjugates dispensing. The antibodies labelled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. This protocol requires only a single ON incubation period at RT.
Assay validation
HSA detection on Primary Human Hepatocytes
Primary Human Hepatocyte supernatants from different single donors (A&B) and donor pool (C&D) were thawed and placed in culture. The supernatants were tested the first day of culture and 4 days after.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target |
HSA
|
| Target Class |
Biologics
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolism/Diabetes
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Human HSA Detection Kit, 500 Assay Points
HTRF Human HSA Detection Kit, 500 Assay Points
Citations
Resources
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Application Note
Multi-parametric high-throughput analysis of hepatotoxicity using a 3D primary liver cell culture model
3D cell cultures offer a more physiologically relevant option, but there are currently only a few high throughput screening (HTS)...
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