Feature | Specification |
---|---|
Application | Protein Quantification |
Dynamic Range | 33 - 10,000 pg/mL |
Limit of Detection | 12 pg/mL |
Sample Volume | 16 µL |
Immunodeficiency Virus (HIV) p24 is the 231 amino acid phosphorylated protein of the capsid forming the conical core of the virus, which encapsulates the genomic RNA-nucleocapsid complex. p24 is a cleavage product of the p55 Gag polyprotein by viral proteases. HIV p24 and its 55 kDa precursor play crucial roles in the assembly, maturation, and disassembly of HIV. e therapy, HIV-derived lentiviral vectors are useful and commonly implemented to deliver transgenes to mammalian cells. Assays against the p24 antigen have become a standard method for measuring lentiviral transduction efficiency.
16 µL of recombinant protein in diluent #5 were dispensed into a low volume white microplate before the addition of 4 µL of the premixed HTRF anti HIVp24 detection reagents. The HTRF signal was recorded after 2h incubation at RT.
Sample size | 16 µL |
Final assay volume | 20 µL |
Time to results | 2h at RT |
Detection limit | 12 pg/mL |
Dynamic range | 33 to 10,000 pg/mL |
Sample compatibility | Cell supernatant |
Application |
Protein Quantification
|
---|---|
Brand |
HTRF
|
Detection Modality |
HTRF
|
Dynamic Range |
33 - 10,000 pg/mL
|
Limit of Detection |
12 pg/mL
|
Product Group |
Kit
|
Sample Volume |
16 µL
|
Shipping Conditions |
Shipped in Dry Ice
|
Target Class |
Biologics
|
Technology |
TR-FRET
|
Therapeutic Area |
Infectious Diseases
|
Unit Size |
500 assay points
|
Are you looking for resources, click on the resource type to explore further.
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
This guide provides you an overview of HTRF applications in several therapeutic areas.
We are here to answer your questions.