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HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
Shipping box for Revvity reagent kits
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 16 µL |
Product variants
Unit Size: 500 assay points
Part #:
64SYN29AGPEG
Unit Size: 10,000 assay points
Part #:
64SYN29AGPEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
Shipping box for Revvity reagent kits
HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
Product information
Overview
Monitoring the phosphorylation and aggregation states of alpha-synuclein is of great interest for studying the pathogenesis of synucleinopathies, a group of neurodegenerative diseases that includes Parkinson's disease (PD), dementia with Lewy bodies (DLB), diffuse Lewy body disease (DLBD), and multiple system atrophy (MSA). The kit provides a solution to rapidly detect and quantify protein aggregates of human alpha-synuclein in cell culture or brain tissue extracts.
How it works
Principle of the HTRF (h) Aggregated Phospho-A-Synuclein Ser129 assay
Alpha-Synuclein aggregates are measured using a sandwich immunoassay involving an anti-a Synuclein monoclonal antibody specific for phospho-Ser129 Alpha-Synuclein aggregates labelled with terbium-Cryptate or d2, ensuring assay quality, reproducibility, and signal quality. The specific HTRF signal generated is proportional to the alpha-Synuclein aggregates.
Protocol of the HTRF Human Aggregated Phospho-A-Synuclein Ser129 assay
The sample or brain extracts are transferred to the assay plate for the detection of Alpha-Synuclein aggregates. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Assay validation
Validation of Aggregated Phospho-A-Synuclein Ser129 detection
A sample generated with aggregated phospho-Alpha-Synuclein (S129) was purified by exclusion phase (G25), resulting in 3 peaks corresponding respectively to oligomers, dimers, and monomers of phospho-Alpha-Synuclein.
Each sample resulting from this purification step was serial-diluted 14 times and plated at 16 µL/well in a low volume white microplate.
2µL of the HTRF d2 detection reagent and 2µL of the HTRF Eu-Tb detection reagent were added, and the signal was recorded after an overnight incubation at RT.
The results show significant detection of the oligomer aggregates only. Closer examinations of the dimer aggregates' detection showed a lower but still detectable signal. Monomers were not detected, demonstrating the specificity of the assay for aggregates only.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Lysis Buffer Compatibility |
Lysis Buffer 5
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
16 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
TR-FRET
|
| Unit Size |
500 assay points
|
Video gallery
HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, 500 Assay Points
Resources
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Brochure
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