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HTRF GST Check Kit, 500 Assay Points
HTRF GST Check Kit, 500 Assay Points
Generic HTRF Anti tag XL665 image
| Feature | Specification |
|---|---|
| Application | タンパク質間相互作用 |
| Sample Volume | 10 µL |
Product variant
Unit Size: 500 assay points
Part #:
62GSTPEG
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF GST Check Kit, 500 Assay Points
Generic HTRF Anti tag XL665 image
HTRF GST Check Kit, 500 Assay Points
Product information
Overview
The kit enables the rapid detection of GST tagged fusion proteins at each step of protein production and purification. It can either be used to ensure that the GST motif of a fusion protein is accessible to the anti-GST antibody, or to approximate the concentration of a GST-tagged protein. Calibrated using the appropriate protein, the assay is very accurate and compatible with the SOPs. It can also be used to eliminate false positives.
How it works
Assay principle
As shown in the graph, GST peptide labeled with XL665 is detected by anti GST Cryptate conjugate (anti-tag Cryptate). The GST-tagged fusion protein (Tag fusion protein) to be ascertained competes with the GST-peptide-XL665 conjugate (Tag-XL665) for binding to the anti-GST-Cryptate conjugate. The specific signal is inversely proportional to the concentration of GST-tagged fusion protein.
Assay protocol
The example on the right describes the protocol using a 20 µL final assay volume. Dispense 10 µL of standard or of GST-tagged protein of interest into a well, with 5 µL of GST Cryptate conjugate and 5 µL of GST-XL665 conjugate. Incubate for 2 hours at RT and read.
Assay validation
Native GST standard curve using GST Check Kit
| Detection limit: | 1 ng/mL (average) |
|---|---|
| S/B | 15 in 96- or 384-well formats |
GST Check Kit performance on various GST-tagged proteins
The GST check kit enables the verification of tag accessibility on GST-tagged fusion proteins. The figure shows the dose response curves of different GST fusion proteins assessed using this kit. All six GST containing proteins were detected using our competitive immunoassay.
Specifications
| Application |
Protein-Protein Interaction
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped Ambient
|
| Target Class |
Binding Assay
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Inflammation
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
500 assay points
|
Video gallery
HTRF GST Check Kit, 500 Assay Points
HTRF GST Check Kit, 500 Assay Points
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