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HTRF Europium Labeling Kit
HTRF Europium Labeling Kit
Generic Vial HTRF
Europium cryptate labeling kit tailored for biomolecule labeling and straightforward development of time-resolved fluorescence resonance energy transfer assays.
| Feature | Specification |
|---|---|
| Application | 標識 |
Europium cryptate labeling kit tailored for biomolecule labeling and straightforward development of time-resolved fluorescence resonance energy transfer assays.
Product variant
Unit Size: 1 kit
Part #:
62EUSPEA
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Europium Labeling Kit
Generic Vial HTRF
HTRF Europium Labeling Kit
Product information
Overview
The Cryptate labeling kit is suitable for quick, easy labeling of peptides, proteins, and oligonucleotides with Europium cryptate. This kit is designed for very small-volume fast labeling needs, such as determining the best antibody among several antibodies available for HTRFassay development.
The N-hydroxysuccinimide activated Europium-Trisbipyridine (TBP) supplied reacts with primary amines carried by the biomolecules to label, for simplified conjugation.
The kit includes all the materials necessary for the labeling procedure (except dialysis and concentration devices).
HTRF assays offer many advantages over other technologies:
- Homogeneous add-and-read format
- No wash steps
- Low background
- Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
- Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
The labeling procedure is performed under very mild conditions. As shown here, the process includes an incubation step with the desiccated dye, followed by purification on a disposable column. Both the purification column and the buffer for purifying the conjugate are included in the kit, as well as a calibrator to define the ideal working dilution of the labeled material in HTRF assays. Depending on the molecular weight of the target molecule and the nature of the dye, each kit enables the labeling of 5-25 µg (protein-MW 15 kDa) to 50-250 µg (antibody-MW 150 kDa).
Assay protocol
The labeling procedure is described on the right. 50 µL of the targeted molecule (6.67 µM) in phosphate buffer pH 8.0 are added to the Europium cryptate vial. After an incubation of 15 min at RT, labeled protein is eluted on an equilibrated column. After having discarded 700 µL of elution buffer, the labeled molecule is collected in 400µL fraction
Specifications
| Application |
Labeling
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped Ambient
|
| Technology |
TR-FRET
|
| Unit Size |
1 kit
|
Video gallery
HTRF Europium Labeling Kit
HTRF Europium Labeling Kit
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Brochure
HTRF assays and reagents product list
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
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