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HTRF CD32a (131H) Cellular Binding Kit, 100 Assay Points
HTRF CD32a (131H) Cellular Binding Kit, 100 Assay Points
Generic HTRF monomer receptor cellular binding image
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Sample Volume | 5 µL |
Product variants
Unit Size: 100 assay points
Part #:
6FRAHPAG
Unit Size: 500 assay points
Part #:
6FRAHPAE
Unit Size: 1,000 assay points
Part #:
6FRAHPAF
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF CD32a (131H) Cellular Binding Kit, 100 Assay Points
Generic HTRF monomer receptor cellular binding image
HTRF CD32a (131H) Cellular Binding Kit, 100 Assay Points
Product information
Overview
The CD32a (FcgRIIa, H131) cellular binding assay is a simple and robust test to monitor binding of the IgG Fc region to the human CD32a receptorin live cells and to select the most potent antibody for use in Antibody-Dependent Cell-Phagocytosis (ADCP). Two separate CD32a assays are available to specifically target the H131 or R131 variants. These assays, based on our innovative Tag-lite technology, can easily be automated to create a high-throughput solution for predicting ADCP.
How it works
Assay principle
The CD32a receptor cellular binding assay is a competitive assay involving HEK293 cells expressing a specific CD32a variant conjugated to Lumi4-terbium Cryptate, and human IgG1 labelled with d2. The unlabeled antibody or Fc tagged drug competes with the d2 labelled IgG1 for binding to the receptor, resulting in a decrease of FRET signal.
Assay protocol
The CD32a receptor cellular binding assays (FcgIIa H131 or R131) present only 1 incubation step after reagents distribution. Lumi4-terbium Cryptate pre-labelled cells are dispensed, followed by the addition of IgG1 labelled with d2 and the sample. After 2h incubation at RT, the signal can be read.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
5 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Biologics
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Rare Diseases
|
| Unit Size |
100 assay points
|
Video gallery
HTRF CD32a (131H) Cellular Binding Kit, 100 Assay Points
HTRF CD32a (131H) Cellular Binding Kit, 100 Assay Points
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Brochure
HTRF assays and reagents product list
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
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