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HTRF cAMP Gs HiRange Detection Kit, 20,000 Assay Points
HTRF cAMP Gs HiRange Detection Kit, 20,000 Assay Points
Generic-HTRF-cAMP-Gs-assay-image
| Feature | Specification |
|---|---|
| Application | Second Messenger Detection |
| Sample Volume | 10 µL |
Product variants
Unit Size: 1,000 assay points
Part #:
62AM6PEB
Unit Size: 20,000 assay points
Part #:
62AM6PEC
Unit Size: 100,000 assay points
Part #:
62AM6PEJ
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF cAMP Gs HiRange Detection Kit, 20,000 Assay Points
Generic-HTRF-cAMP-Gs-assay-image
HTRF cAMP Gs HiRange Detection Kit, 20,000 Assay Points
Product information
Overview
Based on HTRF technology, the kit contains all the reagents needed to quantify cAMP, ensuring that you benefit from a complete tool for your needs.
Revvity's HTRF cAMP kits are best suited for measuring cAMP modulations in response to Gs-activation, leveraging an optimized combination of robustness and sensitivity
How it works
Assay principle
Revvity cAMP kits are based on a competitive format involving a specific antibody labeled with cryptate (donor) and cAMP coupled to d2 (acceptor). This enables the direct characterization of all types of compounds acting on Gs-coupled receptors in either adherent or suspension cells.
Assay protocol
The protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents.
Assay details
cAMP Gs hirange product specifications
If your experimental design uses membrane preparations, lipoparticles or purified receptors, or if your samples have large quantities of cAMP, the Gs HiRange kit will fit your needs!
The kit shows no ATP cross-reactivity up to 10mM, allowing the user to operate with large ranges of ATP and enabling membrane work.
Designed for optimal signal performance, cAMP - Gs HiRange offers a maximized signal-to-background score.
| S/B | IC20 nM | IC50 nM | IC80 nM | |
|---|---|---|---|---|
| cAMP - Gs dynamic | >14 | 0.91 | 4.07 | 18.1 |
| cAMP - Gs HiRange | >28 | 4.27 | 22.8 | 121 |
ATP cross-reactivity
Specifications
| Application |
Second Messenger Detection
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped Ambient
|
| Target Class |
GPCR
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
20,000 assay points
|
Video gallery
HTRF cAMP Gs HiRange Detection Kit, 20,000 Assay Points
HTRF cAMP Gs HiRange Detection Kit, 20,000 Assay Points
Citations
Resources
Are you looking for resources, click on the resource type to explore further.
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
How can we help you?
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