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HTRF EPIgeneous Binding Domain Discovery Kit, 500 Assay Points
HTRF EPIgeneous Binding Domain Discovery Kit, 500 Assay Points
Epigeneous binding domain d2 assay
| Feature | Specification |
|---|---|
| Application | 生化学酵素アッセイ |
| Sample Volume | 120 µL |
Product variant
Unit Size: 500 assay points
Part #:
62BDDPEG
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF EPIgeneous Binding Domain Discovery Kit, 500 Assay Points
Epigeneous binding domain d2 assay
HTRF EPIgeneous Binding Domain Discovery Kit, 500 Assay Points
Product information
Overview
The EPIgeneous Binding Domain kit series provides a simple biochemical approach to study epigenetic reader interactions with modified histones. The three kits, A, B and C, have been validated on 28 different bromo-, chromo- and tudordomains. All kits are based on a GST-tagged binding domain /biotin-coupled Histone peptide format, and can be run using the same add-and-read single plate protocol. Whenever a given domain is not part of the list of validated interactions, the Discovery Binding Domain kit enables researchers to profile which of the A, B or C kits is the best assay solution.
How it works
Assay principle
In EPIgeneous Binding Domain kits, the GST-tagged reader domain protein binding to the biotinylated peptide substrate is detected by a conjugate mix: anti-GST Eu or Tb cryptate-labeled antibody conjugate (donor), and streptavidin conjugated to either d2 or XL665 (acceptor). The interaction of the reader domain with the substrate brings the donor and acceptor dyes into close proximity, and allows FRET to occur upon light excitation. The specific signal at 665 nm is inhibited when a specific compound prevents the reader domain protein from binding to its substrate.
Assay validation
Validated binding domains
Three kits (A, B and C) have already been validated and fully optimized on a selection of 28 key binding domains. For non-validated reader domains, a fourth one, the Discovery Kit, enables researchers to profile which of the A, B or C kits is the best assay solution.
| BINDING DOMAIN KIT A | BINDING DOMAIN KIT B | BINDING DOMAIN KIT C |
|---|---|---|
| BRD2(1) | CECR2 | BRD4(1/2) |
| BRD3(1) | FALZ (BPTF) | ATAD2A |
| BRD4(1) | BRD2(2) | ATAD2B |
| CBX 1 | BRD2(1/2) | BRD9 |
| BRD3(2) | SMARCA4 (BRG1) | |
| BRD3(1/2) | BAZ2B | |
| BRD4(2) | ||
| BRDT(1) | ||
| BRDT(1/2) | ||
| CREBBP | ||
| BRD1 | ||
| BRPF3 | ||
| TAF1L(2) | ||
| TAF1L(1/2) | ||
| TAF1(2) | ||
| TAF1(1/2) | ||
| L3MBTL1 | ||
| UHRF1 |
Specifications
| Application |
Biochemical Enzymatic Assay
|
|---|---|
| Brand |
EPIgeneous
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
120 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
Epigenetics
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Metabolism/Diabetes
Neuroscience
Oncology & Inflammation
|
| Unit Size |
500 assay points
|
Video gallery
HTRF EPIgeneous Binding Domain Discovery Kit, 500 Assay Points
HTRF EPIgeneous Binding Domain Discovery Kit, 500 Assay Points
Resources
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