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HTRF VHH XL665-Conjugate, 5,000 Assay Points
HTRF VHH XL665-Conjugate, 5,000 Assay Points
HTRF MAb Anti Human Kappa-Eu cryptate, 5,000 Assay Points
| Feature | Specification |
|---|---|
| Application | タンパク質間相互作用 |
Product variants
Unit Size: 5,000 assay points
Part #:
610VHHXLA
Unit Size: 20,000 assay points
Part #:
610VHHXLB
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF VHH XL665-Conjugate, 5,000 Assay Points
HTRF MAb Anti Human Kappa-Eu cryptate, 5,000 Assay Points
HTRF VHH XL665-Conjugate, 5,000 Assay Points
Product information
Overview
Monoclonal anti-VHH antibody has been labeled with XL665 and binds to the native form of both alpaca and llama VHH, as well as recombinant nanobodies. The binding is rapid and stable, making it an ideal choice for use in a variety of assays including nanobody selection and titration.
How it works
Assay principle
In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the resulting signal is proportional to the binding of the 2 partners. In the example shown here, anti-VHH-XL665 binds to the nanobodies raised against protein A, while partner A* binds to a specific Ab labeled with an HTRF donor.
*partner A can also be biotinylated, tagged, or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with a donor for the detection.
Assay protocol
The example on the right describes the protocol using a 20 µL final assay volume for the detection of an interaction between a nanobody raised against protein A and a non-tagged partner A*. Dispense the two partners (10 µL): i.e. protein A+nanobody, add anti-partner A labeled with donor (5 µL), and anti-VHH-XL665 (5 µL) Then incubate, and finally read.
*partner A can also be biotinylated, tagged or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin), labeled with a donor for the detection.
Specifications
| Application |
Protein-Protein Interaction
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped Ambient
|
| Target Class |
Binding Assay
|
| Technology |
TR-FRET
|
| Unit Size |
5,000 assay points
|
Resources
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Brochure
HTRF assays and reagents product list
Discover the versatility and precision of Homogeneous Time-Resolved Fluorescence (HTRF) technology. Our HTRF portfolio offers a...
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