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HTRF Anti-VHH Eu-Antibody, 5,000 Assay Points
HTRF Anti-VHH Eu-Antibody, 5,000 Assay Points
HTRF Anti-VHH Antibody, 5,000 Assay Points
HTRF Anti-VHH-Eu cryptate can be used to capture alpaca and llama VHH nanobobies.
| Feature | Specification |
|---|---|
| Application | タンパク質定量 |
HTRF Anti-VHH-Eu cryptate can be used to capture alpaca and llama VHH nanobobies.
Product variants
Unit Size: 5,000 assay points
Part #:
610VHHKLA
Unit Size: 20,000 assay points
Part #:
610VHHKLB
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Anti-VHH Eu-Antibody, 5,000 Assay Points
HTRF Anti-VHH Antibody, 5,000 Assay Points
HTRF Anti-VHH Eu-Antibody, 5,000 Assay Points
Product information
Overview
Monoclonal anti-VHH antibody has been labeled with Eu crypate. Anti-VHH antibody binds to the native form of both alpaca and llama VHH and recombinant nanobodies. The binding is rapid and stable, making it an ideal choice for use in a variety of assays such as nanobodies selection and titration.
HTRF assays offer many advantages over other technologies:
- Homogeneous add-and-read format
- No wash steps
- Low background
- Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
- Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners. In the example shown here, anti-VHH-Eu binds to the nanobodies raised against protein A, while partner A* binds to a specific Ab labeled with an HTRF acceptor *partner A can also be biotinylated, tagged, or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with an acceptor for the detection.
Assay protocol
The example on the right describes the protocol using a 20 µL final assay volume for the detection of an interaction between a nanobody raised against protein A and a non-tagged partner A*. Dispense the two partners (10 µL): i.e. protein A+nanobody, add anti-VHH-Eu cryptate (total 5 µL), and anti-partner A labeled with acceptor (5 µL). Then incubate, and finally read. *partner A can also be biotinylated, tagged, or Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin), labeled with an acceptor for the detection.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Lysis Buffer Compatibility |
Lysis Buffer 1
Lysis Buffer 2
Lysis Buffer 3
Lysis Buffer 4
|
| Product Group |
Antibody
|
| Shipping Conditions |
Shipped Ambient
|
| Target |
VHH
|
| Target Class |
Biomarkers
|
| Technology |
TR-FRET
|
| Unit Size |
5,000 assay points
|
Video gallery
HTRF Anti-VHH Eu-Antibody, 5,000 Assay Points
HTRF Anti-VHH Eu-Antibody, 5,000 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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