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Tag-lite Angiotensin Receptor Red Agonist Ligand
Tag-lite Angiotensin Receptor Red Agonist Ligand
Tube Fluorescent Ligand Red cap
| Feature | Specification |
|---|---|
| Application | 受容体-リガンド結合 |
Product variant
Unit Size: 5,000 assay points
Part #:
L0007RED
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Tag-lite Angiotensin Receptor Red Agonist Ligand
Tube Fluorescent Ligand Red cap
Tag-lite Angiotensin Receptor Red Agonist Ligand
Product information
Overview
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.
This Angiotensin receptor red agonist is an angiotensine II derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Angiotensin AT2 receptor binding assay.
How it works
Assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Specifications
| Application |
Receptor-Ligand Binding
|
|---|---|
| Brand |
Tag-lite
|
| Detection Modality |
HTRF
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target Class |
GPCR
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
|
| Unit Size |
5,000 assay points
|
Video gallery
Tag-lite Angiotensin Receptor Red Agonist Ligand
Tag-lite Angiotensin Receptor Red Agonist Ligand
Resources
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Flyer
Tag-lite: the binding receptor assay portfolio addressing the adenosine receptor family
Tag-lite™ is a cell-based, no-wash alternative to traditional radioisotope assays for studying GPCR-ligand interaction.
Adenosine...
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