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HTRF Aldosterone Detection Kit, 10,000 Assay Points
HTRF Aldosterone Detection Kit, 10,000 Assay Points
Generic HTRF biomarker competition XL665 image
The aldosterone kit is designed for the accurate quantitative measurement of the Aldosterone steroid hormone released by cells.
| Feature | Specification |
|---|---|
| Application | タンパク質定量 |
| Sample Volume | 10 µL |
The aldosterone kit is designed for the accurate quantitative measurement of the Aldosterone steroid hormone released by cells.
Product variants
Unit Size: 500 assay points
Part #:
64ALDPEG
Unit Size: 10,000 assay points
Part #:
64ALDPEH
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Aldosterone Detection Kit, 10,000 Assay Points
Generic HTRF biomarker competition XL665 image
HTRF Aldosterone Detection Kit, 10,000 Assay Points
Product information
Overview
The Aldosterone assay kit accurately measures the release of the Aldosterone steroid hormone in cell supernatant and serum samples. Aldosterone is a steroid hormone synthesized from cholesterol in the adrenal cortex. Aldosterone is metabolized in the kidney and liver, and functions as the key mineralocorticoid in the control of sodium and potassium balance. The Aldosterone kit offers superior benefits over traditional ELISA readouts with the ease of an Add & Read process coupled with the robustness and sensitivity of homogeneous TR-FRET assays.
HTRF assays offer many advantages over other technologies:
- Homogeneous add-and-read format
- No wash steps
- Low background
- Straightforward miniaturization from 96- or 384-well microplates to high density assay formats such as 384-well low volume and 1536-well plates
- Stable signal, providing flexibility in time of readout or size of assays
How it works
Assay principle
The kit is based on a competitive format involving a specific antibody labelled with Cryptate (donor) and Aldosterone coupled to XL665 (acceptor). Aldosterone produced by cells competes with XL665-labelled Aldosterone for binding to monoclonal anti-Aldosterone Eu Cryptate.
Assay protocol
The Aldosterone assay features a streamlined protocol with only two incubation steps: - Cell stimulation by target compounds - Aldosterone detection using HTRF reagents This protocol requires only a single, one-hour incubation period following cell stimulation.
Assay details
Specifications
| Detection limit: | 24 pg/mL |
|---|---|
| Dynamic range: | 0.024 to 160 ng/mL |
| EC50: | 453 pg/mL |
| Specificity: | low cross-reactivity with pregnenolone 0.0003%, progesterone 0.0017%, deoxycorticosterone 0.0017%, corticosterone 0.05% |
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Target |
Aldosterone
|
| Target Class |
Biomarkers
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
|
| Unit Size |
10,000 assay points
|
Video gallery
HTRF Aldosterone Detection Kit, 10,000 Assay Points
HTRF Aldosterone Detection Kit, 10,000 Assay Points
Resources
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Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
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