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Glycan Profiling Assay Reagent Kit

Revvity’s LabChip® Glycan Profiling Assay Reagent Kit enables the high-throughput sizing and quantification of N-linked glycans from protein biotherapeutics on the LabChip® GXII Touch™ protein characterization system.

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Feature	Specification
Instrument Compatibility	LabChip GXII Touch HT
Percent Purity Reproducibility	LMW Protein 30% CV ≤ 80 kDa, HT Glycan: CV < 10% for a peak ≥ 2.5% of total glycans, HT Glycan Extended Range: CV < 10% for a peak ≥ 2.5% of total glycans and at a conc. 2.5 ng/L CV < 25% for a peak ≥ 2.5% of total glycans at conc. 1.0 - 2.5 ng/µL
Sample Volume	8µL with concentration range of 1.25 - 7.5 mg/mL (10 - 60 µg of MAb total)
Size Range	HT Glycan: neutral glycans found on mAbs, some charged glycans may run outside of our usable range HT Glycan Extended Range: neutral and charged glycans found on mAbs
Sizing Precision RSD (CV)	CV < 2.5%
Technology	マイクロ流体電気泳動

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Product variant

Sample Type: グリカン

Part #:

760525

For research use only. Not for use in diagnostic procedures.

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Product information

Overview

Glycan profiling and screening, super charged.

A high percentage of all biotherapeutics contain N-linked glycosylation. The impact of N-linked glycosylation on protein function can be significant. Thus, the characterization of N-glycan composition is critical for the biotherapeutic development process. The LabChip® Extended Range N-Linked Glycan assay provides a fast, efficient means to assess the main glycoforms of IgG and related proteins.

Glycoprotein Sizing, Glycoprotein Relative Quantification & Charge Variant N-Glycan Analysis

The LabChip® Glycan Profiling Assay Reagent Kit is ideal for rapid screening of N-linked glycan profiles by combining:

On-plate protein denaturation
Rapid enzymatic deglycosylation with PNGase F in 96 well format
Rapid electrokinetic separations and detection using LabChip® microfluidic chip technology
Per sample run time is under 45 seconds with a full 96 sample analysis in under 90 minutes
Automated peak detection and reporting of N-glycan relative abundance
Minimal sample consumption: Only 8 µL of sample required
Digital data format: Gel-like image, electropherogram, and tabular data formats with automated sample alignment, overlay, scaling and zooming features, allow users to conveniently share, store and archive data
The Glycan Profiling Assay Reagent Kit supports both neutral glycan and charged N-linked glycan found in MAbs. Drop down menus allow you to easily choose between assays.
To release and label glycoforms of IgG and related proteins, use the Glycan Release and Labelling Kit, before analyzing with the Glycan Profiling Assay Reagent Kit

Additional product information

labchip extended range n glycan assay image 1

labchip extended range n glycan assay fig1

Figure 1: The LabChip Glycan Profiling Assay Reagent Kit supports the sizing and quantification of N-linked glycans in concentrations from 1.25 ng/µl (left) to 7.5 ng/µl (right)

Specifications

Other specifications

Application	Protein Analysis
Automation Compatible	Yes
Instrument Compatibility	LabChip GXII Touch HT
Percent Purity Reproducibility	LMW Protein 30% CV ≤ 80 kDa, HT Glycan: CV < 10% for a peak ≥ 2.5% of total glycans, HT Glycan Extended Range: CV < 10% for a peak ≥ 2.5% of total glycans and at a conc. 2.5 ng/L CV < 25% for a peak ≥ 2.5% of total glycans at conc. 1.0 - 2.5 ng/µL
Sample Type	Glycan
Sample Volume	8µL with concentration range of 1.25 - 7.5 mg/mL (10 - 60 µg of MAb total)
Size Range	HT Glycan: neutral glycans found on mAbs, some charged glycans may run outside of our usable range HT Glycan Extended Range: neutral and charged glycans found on mAbs
Sizing Precision RSD (CV)	CV < 2.5%
Shipping Conditions	Shipped in Blue Ice Soft
Technology	Microfluidic Electrophoresis
Unit Size	1 unit

References

Loebrich, S., Clark, E., Ladd, K., Takahashi, S., Brousseau, A., Kitchener, S., Herbst, R., & Ryll, T. (2018). Comprehensive manipulation of glycosylation profiles across development scales. MAbs, 11(2), 335–349. doi.org/10.1080/19420862.2018.1527665.