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HostDetect HEK293 PCR DNA Quant Kit
HostDetect HEK293 PCR DNA Quant Kit
HostDetect HEK293 PCR DNA Quant Kit
| Feature | Specification |
|---|---|
| Analytical Sensitivity | > 0.03 pg/rxn |
| Linear Concentration Range | R2 ≥ 0.99, Eff%= 100±20% |
| Sizing Precision RSD (CV) | CV < 5% at 0.1 pg/rxn |
Product variant
Unit Size: 192 rxns
Part #:
DXMDX-RGT-1004
For research use only. Not for use in diagnostic procedures.
HostDetect HEK293 PCR DNA Quant Kit
HostDetect HEK293 PCR DNA Quant Kit
HostDetect HEK293 PCR DNA Quant Kit
Product information
Overview
The HostDetect HEK293 PCR DNA Quant Kit utilizes sequence-specific primers and TaqMan® probe to amplify the Alu-equivalent gene of HEK293 genomic DNA for residual host DNA identification. A primer/probe set to detect Internal Control (IC), is also included for monitoring entire process from extraction to real-time PCR. The reagents also use a dUTP/UNG carryover prevention system to avoid contamination of PCR products.
This kit is specific for DNA from the HEK293 genome and cannot detect human or environmental DNA that might be introduced during sample handling.
For more information, contact: BioprocessQC@revvity.com
Key highlights:
- Quantify HEK293 genomic DNA with as little as 0.03 pg per PCR reaction in < 2 hours of PCR
- Internal control detection for process monitoring from sample extraction to PCR
- High specificity: No cross-reactivity with unrelated DNA
- Flexible extracted DNA input volume up to 10 µL per well
- Scalable reaction numbers from 1 to up to 192 per kit
Additional product information
HostDetect™ HEK293 PCR DNA Quant Kit Workflow
The probes for the HEK293 genomic DNA and Internal Control detection are labeled with FAM and HEX/VIC fluorescent dyes, respectively, to generate target-specific signal. ROX is used for Passive Reference. Any Real-time qPCR Instruments with FAM™ and VIC®/HEX™ channels can be used with this assay.
HostDetect™ HEK293 PCR DNA Quant Kit Quantification Range**
**: ATCC or USP reference standards for residual DNAs are not included in the kit. Customer must order these separately from ATCC or USP web sites.
Features:
| Quantification Range | 1 ng to 0.1 pg/rxn |
|---|---|
| Linearity | R2 ≥ 0.99, Eff%= 100±20% |
| Sensitivity | ≥ 0.03 pg/rxn |
| Precision | CV < 5% at 0.1 pg/rxn |
| Shipping Conditions | Shipped on Dry ice |
| Storage Conditions | -25 to -15 °C |
Specifications
| Analytical Sensitivity |
> 0.03 pg/rxn
|
|---|---|
| Limit of Quantification |
1 ng to 0.1 pg/rxn
|
| Linear Concentration Range |
R2 ≥ 0.99, Eff%= 100±20%
|
| Sizing Precision RSD (CV) |
CV < 5% at 0.1 pg/rxn
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Storage Conditions |
-25C to -15C
|
| Unit Size |
192 rxns
|
Resources
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Application Note
Monitoring residual HEK293 DNA in AAV-based therapies using the HostDetect HEK293 PCR DNA quant kit
"Residual HEK293 DNA is a critical impurity that must be minimized when generating recombinant AAV (rAAV) therapeutic products, as...
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