HostDetect E.coli PCR DNA Quant Kit

The HostDetect E. coli PCR DNA Quant Kit utilizes sequence-specific primers and TaqMan® probe to amplify the 16S ribosomal RNA gene of the E. coli genome for residual host DNA identification. A primer/probe set to detect Internal Control (IC), is also included for monitoring entire process from extraction to real-time PCR. The reagents also use a dUTP/UNG carryover prevention system to avoid contamination of PCR products.

This kit is specific for DNA from the E. coli genome and cannot detect human or environmental DNA that might be introduced during sample handling.

For more information, contact: BioprocessQC@revvity.com

Key highlights:

• Quantify E.coli genomic DNA with as little as 0.03 pg per PCR reaction in < 2 hours of PCR
• Internal control detection for process monitoring from sample extraction to PCR
• High specificity: No cross-reactivity with unrelated DNA
• Flexible extracted DNA input volume up to 10 µL per well
• Scalable reaction numbers from 1 to up to 192 per kit